痘病毒

Lee for the Department of the skin of that, at the beginning that women Roufan lesion is fungal infection, a lesion in the testing organization, under the microscope, they found that the oval-shaped ball of the smallpox virus, it is tenacious , Dry-resistant, high temperature and organic solvents, but also contaminated feed trough and drinking water trough.

台北荣民总医院皮肤部主治医师李定达说，一开始以为女肉贩病灶是霉菌感染，在取得病灶组织化验后，在显微镜下，却发现了椭圆形球状的副痘病毒，它极顽强，耐乾燥、高温及有机溶剂，还会污染饲料槽和饮水槽。

Selective induction of immune responses by cytokines coexpressed in recombinant fowl- pox virus .

表达禽流感HA和NP双基因重组禽痘病毒的免疫保护性。

Methods Using prime-boost immunization strategy,BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus.

目的：探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒，以肌肉注射的方式免疫BALB／c小鼠，ELISA法检测小鼠血清HIV-2抗体，流式细胞仪测定CD4^＋、CD8^＋T淋巴细胞亚类数量，乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

From the cattle and sheep pox virus deputy, in a red thumb,"milking people nodules."

来自牛羊的副痘病毒，在大拇指出现红色的「挤奶人结节」。

Li set up, butcher, Roufan, veterinarians, milking, farm workers or animal husbandry is the high infection ethnic groups; Some patients regardless of it, so that their wounds, and only if the infection of cattle and sheep pox virus, may not have died of eating , Lactation or less.

李定达说，屠夫、肉贩、兽医、挤奶人、农场工人或畜牧业者是高感染族群；也有的病人不管它，让伤口自己好，牛羊只若感染副痘病毒，有可能不进食而死亡，或泌乳量减少。

Full genome of HCV ; Clone ; Transfected cell culture system ; Recombinant vaccinia virus

HCV全基因组；克隆；转染细胞体系；重组痘病毒

The aim of this study is to evaluate the genetic stability of the recombinant fowl poxvirus expressing E0 and E2 gene of Shimen strain of classical swine fever virus.

研究共表达猪瘟病毒E0、E2基因的重组鸡痘病毒rFPV-E0-E2的遗传稳定性。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

For instance, in 2003 an outbreak of monkeypox affected around 20 people in the US midwest, traced to imported Gambian pouched rats from Africa.

2003年，美国中西部地区的约20人感染了一种&猴痘&病毒，这种病毒源于从非洲进口的一批冈比亚巨鼠。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。