痘

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

The earliest vaccines were made from liquid from the pocks of cattle.

最早的疫苗是用牛的痘浆制成的。

But my translation is so slow, they take child and smell and begin to - interject: the smell of Calomine when he had chicken pocks, the smell of the baby's breath after nursing….

但是我的翻译太慢，他们一接过孩子和气味，就开始插话：他出痘时卡罗敏止痒霜的气味，婴儿刚喂过奶后呼吸的气息。

To mark with pocks; pit .

使有痘痕；使有疤痕

After the dragon boat festival and entering the middle of June, it is unacceptable to us for the hot weather. I can't image the feeling for just 2 month little baby. Today there are two more pocks were produced on the top of head. I am afraid that my cute daughter will become ugly face.

过了端午节进入了6月中旬，天气越来越热令人受不了，何况是刚出生2个月的婴儿，今天再度在金柏妘的头顶上，看到2颗痘子跑出来，很害怕可爱的小妹妹变成大花脸，希望能找到降火气的方法。

After the dragon boat festival and entering the middle of June, it is unacceptable to us for the hot weather. I can't image the feeling for just 2 month little baby. Today there are two more pocks were produced on the top of head. I am afraid that my cute daughter will become ugly face. I hope to find the way to reduce the internal heat on her body.

过了端午节进入了6月中旬，天气越来过了端午节进入了6月中旬，天气越来越热令人受不了，何况是刚出生2个月的婴儿，今天再度在金柏妘的头顶上，看到2颗痘子跑出来，很害怕可爱的小妹妹变成大花脸，希望能找到降火气的方法。

The one on my right eyebrow, for example, and acouple of ancient pockmarks and starbursts on my knees.

比如我右眼眉毛上的那一块，还有膝盖上那些很久以前留下的痘点和放射状疤痕。

The one on my right eyebrow, for example, and a couple of ancient pockmarks and starbursts on my knees.

比如我右眼眉毛上的那一块，还有膝盖上那些很久以前留下的痘点和放射状疤痕。

The one on my right eyebrow, for example, and a couple of ancient pockmarks and starbursts on my knees.

比如，右眉上的那个疤，几个很久以前留下的痘痕，还有膝盖上星星点点的疤痕。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。