病理情况

The result show in transplanted vessel EF-1 and PCNA are mainly expressed in smooth muscle cells and Clemons rate proliferation of SMC is the core of endothelium proliferation.In the third part of our experiment we use fluid nitrogen to fire transplanted rein, crofter tissue homogeneous to test the expression of CE and EF-1 gene we use RT-Craned immunohistologiccal method to test mKNA and protein level respectively and analyze the expression of these tm genes in transplanted vein.

本实验的第二部分，我们将术后大鼠分为10组，每组8只，分别于术后6hours、24hours、72hours、1W、10days、2W、3W、4W、6W、8W切取移植静脉，将切取的不同时期的移植静脉分别置于10％中性福尔马林溶液中固定和液氮冷冻固定，将福尔马林溶液固定的标本制成4μm厚石腊切片，，常规进行病理观察大体改变，静脉标本HE染色后于光镜下观内膜增殖情况，并应用PCNA单克隆抗体，观察SMC增殖情况。

Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法：病理组织切片以CD34单克隆抗体作为第一抗体，按照免疫组织化学SP步骤进行，DAB显色，苏木素复染，每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司)，应用免疫组化显示血管，对不同时期的血管生成情况及早期的炎性反映情况进行观察。

Methods: Mixed bacterium was used for making salpingitis caused sterility rats model, treated by different doses of TLF contrasted with Fuyankang , the pathology and morphallaxis of oviduct with Light and Transmission electron microscope was observed, the content of IL-2、IL-6 in serum and oviduct was checked by ELISA method, pregnant results also was traced.

用"混合菌接种法"制作大鼠慢性输卵管炎性不孕动物模型，以不同剂量的通络助孕方和对照药妇炎康对模型大鼠治疗后，观察光镜和电镜下输卵管病理形态学改变情况，用ELISA法检测血清和输卵管组织中IL-2、IL-6含量，并观察大鼠受孕情况。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

We analyzed 120 ampullary adenomas (31 pure adenomas and 89 carcinoma-associated adenomas) and 170 pure adenocarcinomas for MSI, immunohistochemical expression of MMR proteins and specific histopathologic features.

我们分析了120例壶腹部腺癌（31例纯腺癌、89例腺瘤伴癌变）和170例纯腺癌的MSI情况、MMR蛋白免疫组化的表达情况及其组织病理特征。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Methods: Integrated and disrepaired skins in the rats were smeared with Salicylic AcidPhenol Liniment by the maximum tolerated dose only. The toxic reaction of the animals were observed and recorded continuously from the beginning of using the drug to 7 days, and evaluated by using the general standard of the reaction of skin stimulation in pharmacology. Animals were executed by taking off acanthi and blood was collected to check up the periphery blood routine and serum biochemistry at the end of the observation of the rats. And after the dissection, the pathological change of main visceras, such as heart, brain, liver and kidney were observed with naked eye and pathological slices. The data of this experiment was used to calculate the value of LD50 by using Bliss rule.

观察大鼠完整皮肤及破损皮肤一次性涂抹最大耐受量的小儿水杨酸苯酚搽剂所产生的毒性反应情况，从给药0时起至7 d连续观察记录动物的急性毒性和45 d连续的长期毒性反应，以皮肤刺激反应标准进行评分作毒理等级评价；对存活动物在观察结束时进行高位脱椎处死采血检测外周血常规和血清生化，解剖后裸眼和病理切片观察心、脑、肝、肾等主要脏器的病理变化。

Haematoxylin-eosin staining specimens were observed with light microscope. So as for pathological changes of angle of anterior chamber, trabecular meshwork, ciliary body, choroids, retinal ganglion cell and layer of optic fibers. TUNEL technology was used to determineapoptosis of RGCs. Value of uveoscleral outflow was measure with FITC-Dextran as labeled tracer. Results:I.

直接眼压计测量眼压；常规HE染色光学显微镜下观察前房角、小梁网及睫状体的病理改变，观察脉络膜、视网膜神经节细胞及视神经纤维层病理改变；TUNEL法检测视网膜神经节细胞的凋亡情况；利用FITC-Dextran作为标记物测定葡萄膜巩膜引流途径房水流量。

These animals were killed after 24 hours of treatments and instillation of meconiums. The measurements included lung tissue wet/dry ratio,lung injury score,the expression and level of AngⅡ,lung myeoperoxidase activity and TNF-α level.

模型制备后24h取材，观察其肺组织病理损伤并检测湿/干重比及进行病理评分；检测AngⅡ在肺组织的表达情况及用图像分析方法检测其含量；测定肺组织中肿瘤坏死因子-α的含量及肺匀浆髓过氧化物酶活性。

Objective To survey the prevalence of Helicobacter pylori in gastrointestinal out-patient department in Han group in Hainan. Methods There 2 395 patients from ten counties or cities in Han group in Hainan at gastrointestinal out-patient department of the hospital were detected by rapid urease test of H.pylori and gastroscope. The posititives of rapid urease test of H.pylori were determined with 14C-urea breath test (14C-UBT).Two positive tests were confirmed to be infected with H.pylori. Biopsy was performed on 1 004 patients positive for H.pylori. 113 healthy people were enrolled in control group.

目的 调查海南汉区在消化内科就诊患者中幽门螺杆菌（Helicobacter pylori，H.pylori）感染患病情况，以及幽门螺杆菌感染与胃黏膜病理的关系方法选择海南汉区10 县市2 395例具有上消化道症状就诊患者，经胃镜检查并取胃窦黏膜做H.pylori快速尿素酶试验，阳性患者做14C呼气试验，两者阳性确定幽门螺杆菌感染。1 004例幽门螺杆菌阳性患者取胃窦及病变部位胃黏膜做病理组织检查。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。