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In this research, the isolates of TuMV from Shandong were separated and purified. Their biological, serological, cytopathological and particle morphological properties were studied.

本研究分离纯化了芜菁花叶病毒的山东分离物,对其生物学、血清学、细胞病理学和粒子形态学特征进行了研究。

Cytopathological study on leaves of pumpkin infected with PRSV-W, WMV showed that there were large amount of inclusion in shape of pinwheel, lamina, and braid in the cells, and there were many linear virus particles fascicled or scattered in the cytoplasm, causing the cytopathological characters which was similar to typical potato virus Y .

对PRSV-W、WMV侵染的南瓜病叶的细胞病理学观察结果表明,在感病细胞中有大量的风轮状、片层状和条带状内含体,线状病毒粒体呈束状或散布在细胞质中,具有典型的马铃薯Y病毒属侵染寄主引起的细胞病理特征。

It was negative for co-culture of Vero cells with upper fluid of iris, ciliary body and retina which were blindly transfered to the third generation. No Cytopathological effects were observed, while the positive contrast group had CPE including such as ballooning degeneration, karyopyknosis ,multinucleated giant cell even cell necrosis.

Vero 细胞分别与虹膜、睫状体、视网膜组织上清液共培养,并盲传三代结果为阴性,未观察到细胞病理学改变,对照组有阳性表现,表现为Vero 细胞气球样变、核固缩、多核巨细胞甚至细胞悬浮死亡。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征;利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类;对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增,克隆到pGEM-T载体并进行测序;应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

The dags in experimental group were performed with anastomosis of intestine by using medical adhesive,while the dogs in control group were treated by using traditional modus operandi.

将分别测定两种吻合方法的操作时间、吻合口通畅程度、吻合口爆裂压、吻合口羟脯氨酸含量及标本组织病理学比较。

Hepatocyte damnify, adipohepar and fiber tissues of liver in the treatment group were fewer than model group.

病理学提示,治疗组肝细胞损伤、肝脏脂肪变性与纤维组织形成较少。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各组海马区脑损伤行组织病理学检测,半胱氨酸天冬氨酸蛋白酶-3 mRNA的表达采用半定量反转录-聚合酶反应方法进行检查,成年90 d龄的大鼠进行三等分迷宫测试其学习记忆能力,3个实验各自独立。

With the increasing knowledge in this arena, complete decongestive physical therapy was firstly developed in Europe and was introduced later to the States for patients with lymphedema.

随著对淋巴系统的解剖构造以及生理病理学的了解逐渐增加,完整之减肿胀物理治疗法是一套最早在欧洲被提出来治疗淋巴水肿病人的方法,现今除了欧洲之外亦被广泛用於美国。

With the increasing knowledge in this arena, complete decongestive physical therapy was firstly developed in Europe and was introduced later to the States for patients with lymphedema.

隨著对淋巴系统的解剖构造以及生理病理学的瞭解逐渐增加,完整之减肿胀物理治疗法是一套最早在欧洲被提出来治疗淋巴水肿病人的方法,现今除了欧洲之外亦被广泛用於美国。

Of the approaches the dentinal caries cleaning considered in this article only the reduction of dentinal caries removed seems to have been satisfactionally established on the basis of the histopathologic considerations and clinical observation.

於本文中,对牙本质龋齿层,在组织病理学上及临床上的观察都有令人满意果。

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