病毒病

Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒，并对阳性病料做病毒分离与纯化。

Background Evidences, from recent studies, suggested that Borna disease virus infection might be associated with human neuropsychosis, especially psychiatric disorders including depressive disorder.

背景 博尔纳病病毒(Borna disease virus，BDV)是一种高度嗜神经的RNA病毒，是人畜共患病博尔纳病(Borna disease，BD)的病原体，可引起从鸟到灵长类的多种动物的中枢神经系统感染[1]，表现为以中枢神经系统功能障碍为特征的BD。

With in situ DNA hybridization, the EBV genomes have been demonstrated in the labial glands of the Sjogren′s syndrome patients (16/28), moreover the elevated content of EBV DNA has been identified in those with more severe destruction of labial glands. It is concluded that EBV, vhich has a normal site of latency at salivary glands in a nonpathogenic state, may be reactivated in Sjogren′s syndrome patients and play a role in the pathogenesis of this disease.

另外，我们用原位杂交方法观察了EB病毒DNA在干燥综合征病人唇腺组织的存在状况，结果发现28例干燥综合征病人中16例为阳性，其中腺泡组织破坏严重者，EB病毒DNA的含量亦高，并且EB病毒主要分布于大量浸润的淋巴细胞中，其次位于腺泡上皮细胞内，少量见于腺泡腔中，以上结果提示，在干燥综合征病人EB病毒处于活跃的复制状态，干燥综合征病人靶器官受损与EB病毒的活跃复制及其诱导的免疫反应密切相关。

We established a stable expressing system of BDV phosphoprotein in PC-12 cell. This cell model can be used to study the effect of BDV phosphoprotein on the centre nervous system without exposure to live virus.

此外本文通过检测细胞的增殖活性发现博尔纳病病毒磷蛋白对PC-12细胞的生长具有明显的抑制作用，可能是博尔纳病病毒持续感染中枢神经系统的重要机制之一。

Objective? To establish stable expressing system of Borna disease virus phosphoprotein in PC-12 cells, and then study its influence on cell proliferation of PC-12 cells.?Method?

建立博尔纳病病毒磷蛋白在神经源性PC-12细胞内的稳定表达体系，初步探讨博尔纳病病毒磷蛋白对PC-12细胞的生长是否有影响。

Aphids in the process of inhaling plant sap virus particles and auxiliary proteins also be inhaled, thus completing the process by poison, which also supports the field citrus aphid is non-persistent decline disease vector of disease, while the citrus Huanglongbing is phloem restricted by the bacteria caused the main pathogens present in the phloem, the fleas only by phloem feeding in diseased plants, pathogens in the body through the back to complete the salivary glands in the course of the next through the sieve tube feeding in the secretion of saliva the pathogens into the healthy tree, Citrus Huanglongbing different characteristics with aphid-borne diseases, Huanglongbing to persistent disease.

蚜虫在吸入植物汁液的过程中病毒粒子和辅蛋白同时也被吸入，从而完成获毒过程，这也支持了田间蚜虫是柑桔衰退病等非持久性病害传播介体，而柑桔黄龙病是由韧皮部限制性细菌类引起，病原体主要存在于韧皮部中，木虱只有通过在病株韧皮部取食，病原在体内完成循回到唾液腺中，在下一次取食过程中通过在筛管中分泌的唾液将病原体带到健树，柑桔黄龙病与蚜虫传病特点不同，黄龙病为持久性病害。

A multiplex RT-PCR was successfully established to simultaneously detect Citrus Huanglongbing pathogen ( Candidatus Liberibacter asiaticus, HLB), Citrus exocortis viroid and Citrus tristeza viru s. Using three sets of specific primers designed according to the converted sequences of these three

建立了一种同时检测柑橘黄龙病病原类细菌( Candidatus L iberibacter asiaticus， HLB)、柑橘裂皮类病毒( Citrus exocortis viroid ， CEVd)、柑橘衰退病病毒( Citrus tristeza virus ， CTV)的多重RT-PCR技术体系。

Cytopathological study on leaves of pumpkin infected with PRSV-W, WMV showed that there were large amount of inclusion in shape of pinwheel, lamina, and braid in the cells, and there were many linear virus particles fascicled or scattered in the cytoplasm, causing the cytopathological characters which was similar to typical potato virus Y .

对PRSV-W、WMV侵染的南瓜病叶的细胞病理学观察结果表明，在感病细胞中有大量的风轮状、片层状和条带状内含体，线状病毒粒体呈束状或散布在细胞质中，具有典型的马铃薯Y病毒属侵染寄主引起的细胞病理特征。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料，通过9～11 日龄非免疫鸡胚进行了病毒的实验室分离和传代，并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定，结果表明，4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞，标准阳性血清可特异性的抑制其凝集性，回归试验可复制出与自然发病相同的病例，病毒传代物有明显的致鸡胚矮小化作用，透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子，将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

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舒美公司的&舒安美&系列消毒剂可有效杀灭各类细菌（含MRSA， VRE等耐药菌）、真菌、病毒（如：H1N1流感病毒、手足口病病毒、禽流感病毒、SARS病毒、爱滋病病毒等）和各类导致医院感染爆发的棘手细菌（如：鲍曼不动杆菌、艰难芽孢梭菌等），为您提供安全、高效、全面的保护。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。