病毒

Key words: recombinant adenovirus;pseudorabies virus;gC protein;monoclonal antibody

重组腺病毒；伪狂犬病病毒；gC蛋白；单克隆抗体

The purpose of this research is to generateengineering antibody by using phage display technology,and to investigate its binding activitywith the pseudorabies virus.

本研究的目的就是克隆抗伪狂犬病病毒抗体重链、轻链可变区基因，构建单链抗体基因，利用噬菌体展示技术制备噬菌体抗体，并在大肠杆菌中进行可溶性表达，获得具有对伪狂犬病病毒结合活性的表达产物，为以后在治疗、预防上的应用奠定基础。

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。

EXE file in the Windows system directory, writes its "pure" code to there and runs this file.

EXE的文件，写入病毒体本身，并运行之，这个病毒的拆离体

This virus can point to the IE browser of the user the advertisement webpage that appoints to virus author, return the information of meeting purloin user at the same time.

该病毒会将用户的IE浏览器指向到病毒作者指定的广告网页，同时还会盗取用户的信息。

This virus is program of an advertisement spy, it can point to the IE browser of the user the advertisement webpage that appoints to virus author, return the information of meeting purloin user at the same time.

该病毒是一个广告间谍程序，它会将用户的IE浏览器指向到病毒作者指定的广告网页，同时还会盗取用户的信息。

The method was successfully used to quantitate the virus load in tiss...

最后用建立的方法对108份临床样品进行检测并同时进行病毒分离，荧光定量PCR方法检测出73份阳性样品且与病毒分离的符合率为100%，而常规RT-PCR只检测出54份阳性样品，表明本荧光定量RT-PCR法在检测猪瘟病料上具有潜在的应用价值。

To develop a rapid and accurate method for baculovirus titration, flow cytometry was adopted to quantitate baculovirus directly after staining with SYBR Green I.

为了快速而准确的测定杆状病毒的滴度，采用流式细胞术对经SYBR Green I染色后的杆状病毒直接计数。

Was inoculated into chenopodium quinoa to reproduce in large number .Then the virus was extracted and purified for observing by electronic microscope and sequencing.

将从樱桃树上分离的得到的病毒，接种的藜科的茴菜Chenopodium quinoa上，大量繁殖，然后提纯病毒进行电镜观察和核酸序列测定。

Methods: Bacterial infection mouse models were established by the peritoneal injection of Staphylococcus aureus and viral pneumonia mouse models by nose drip of influenza virus; Fever rabbit models were produced by vein injection of indotoxin. YZHH was used to treat these animal models.

采用小鼠腹腔注谢金黄色葡萄球菌的方法观察一枝黄花抗感颗粒的体内抗菌作用；采取流感病毒滴鼻致小鼠流感病毒性肺炎模型观察其对小鼠的抗病毒作用；采用家兔耳缘静脉注射内毒素致热的方法，观察其解热作用。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。