病毒

Many studies revealed that pokeweed antiviral protein can control plant virus effectively.

许多研究发现美洲商陆抗病毒蛋白(pokeweed antiviral protein，PAP)对植物病毒具有良好的控制效果。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Any of a group of enteroviruses that can cause a disease resembling poliomyelitis but without paralysis.

库克萨基病毒任一种肠道病毒，能引起以脊髓灰质炎为特征的疾病，但不会导致瘫痪

The number of polyhedral inclusion bodies was increased 32.3% and the titer of extracellular virus was increased 20.9%.

在换用的培养液中添加1％TPB可以提高病毒产量，多角体产量增加32.3％，游离病毒粒子的滴度提高20.9％。

The results from the digestion by restriction enzymes showed that there were less EcoR I sites in genomic DNA than in that of Bombyx mori nuclear polyhedrosis virus. It suggested that there might be a different viral replication mechanism in ApNPV life cycle.

结果表明，在ApNPV基因组中只含少量的EcoR I位点，与家蚕核型多角体病毒差别很大，并提示这两种病毒的复制机制可能不同。

Bombyx Morinuclear Polyhedrosis Virusis a kind of circular DNA virus.

家蚕核型多角体病毒是一类环状DNA病毒。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白，利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白，包括原核和真核基因编码的蛋白。

A polymorphic virus can also belong to any of the virus types discussed above.

具有多形态的病毒也可属于以上讨论的任何一种病毒。

CMV infections were not significantly reduced with either polyvalent IVIG or CMV-IVIG.

使用多价免疫球蛋白或巨细胞病毒免疫球蛋白都未能显著降低巨细胞病毒的感染。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。