病毒

Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

It appears in Zhang Linqi, HIV-positive pregnant women, even prior to the delivery, if timely intervention of drugs, AIDS continues to be mother to child transmission probability from 30% to 15%, as the most crucial is to reduce the mother of the AIDS virus in the body, Therefore, early detection, early treatment will bring about the blocking effect of high-quality,"just down the mountain to block HIV, is slowly declining, through medication, at least two weeks to a buffer in order to allow HIV-positive pregnant women in the body The AIDS virus from high to very low levels of inhibition."

在张林琦看来，HIV阳性的孕妇即便到了分娩之前，如若及时进行药物干预，依然能将艾滋病母婴传播几率从30%降低至15%左右，因为最为关键的是降低母亲体内的艾滋病病毒量，故而，早发现、早治疗会带来优质的阻断效果，&阻断艾滋病病毒就跟下山一样，是个慢慢下降的过程，通过服药，至少需要两周时间缓冲，才能让HIV阳性的孕妇体内的艾滋病病毒从很高水平抑制到很低水平&。

In this study, a reverse genetic system was tried to establish for the avian influenza virus A/Goose/Guangdong/1/96 (H5N1) and for the Borna disease virus respectively.

A型流感病毒能够感染哺乳动物和各种鸟类，其中禽流感病毒已经给世界养禽业造成巨大损失，同时也是人类健康潜在威胁。鲍纳病毒能够感染多种温血动物，并可造成感染动物中枢神经系统持续感染，导致神经系统疾病。

It brings a lot of damages to chicken farming.

MDV(Marek's disease virus)是疱疹病毒科的一种细胞结合性肿瘤病毒，严重危害养鸡业的发展，且该病毒毒力呈增强趋势。

The total RNA of viruses isolated was extracted from the Hantavirus-infected Vero-E6 cells and the S-segment cDNA of Hantavirus was obtained by RT-PCR. This gene fragment was then cloned into plasmid vector pUCm-T and sequenced afterwards by using the DNA STAR software for comparison.

提取病毒总RNA，应用逆转录聚合酶链反应扩增病毒S基因全片段，克隆入质粒载体，进行核苷酸序列测定及分析，应用DNA STAR软件分析比较，确定病毒型别。

Removal of virus-enriched culture fluid Virus growth and release is generally accompanied by cell degeneration, so that at the time virus replication is complete, most of the cells in the culture have become converted into cell debris.

富含病毒培养液的去除。病毒的生长和释放伴随着细胞的破损。在病毒复制完成的时候，培养液中大多数细胞已经变成了细胞碎片。

METHODS: Using tissue culture technique, the maximal non-toxic concentration of luteolin in swine testicle cell culture was measured, and the inhibitory effect of luteolin on TGEV was measured by cytophathogenic effect observation and crystal violet measurement. In addition, the degrees of inhibition of the luteolin on CPE of virus in cell culture were observed by changing sequence of adding the vires and the drag. RESULTS : The maximal non-toxic concentration of luteolin was 873.39 μmol/L.

采用细胞培养法，通过结晶紫摄入量测定结合细胞病变作用，观察木犀草素在不同的加药方式下，对感染TGEV的猪睾丸细胞的保护作用、结果：TGEV病毒先吸附ST细胞再加入药液，先与TGEV病毒孵育再接种于卯细胞，或药液先与细胞孵育再用病毒感染等不同方式下，木犀草素在0.84-54.50μmaol／L浓度范围内，均能明显地提高细胞的存活数。

In addition, it was also found that the sulfated α-D-1, 3-glucans with higher degree of substitution had suppressive effects on dimethylbene-induced ear shell swell of mice, and possessed antivirus (HSV-1 and Influenza A) activities in vitro, suggesting that the sulfated glycans have potential usages in the release of inflammation of nerve system and the infection of some virus.

另外，还发现，具有较高取代度的硫酸化灵芝多糖对二甲苯诱导的小鼠耳壳肿胀，单纯疱症病毒（HSV-1）对Vero细胞的感染及A型流感病毒对MDCK细胞的感染均有一定的抑制作用，说明硫酸化灵芝多糖对神经系统炎症及病毒感染具有潜在的治疗作用。

Conway and co-workers used cryo-em to study the conformations of virus capsids as the matured over time, a process that results in the expansion and increased rigidity of the viral shell.

Conway 和同事使用了低温－电子扫描以研究病毒病毒衣壳过成熟后的组成，这个过程导致了此病毒壳体膨胀和僵硬度增强。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。