病毒

The second time occurred in June, 1999 which was caused by Pan-Asia topotype serotype O FMDV (O/Taiwan/99). The virus was firstly isolated from Chinese yellow cattle in Kinmen and could infect goats and pigs.

第二次发生在1999年6月，是由泛亚洲O血清型口蹄疫病毒(O/Taiwan/99)所造成，该株病毒最早是从金门的黄牛所分离到，该株病毒除了感染牛只之外，也会造成羊只及猪只的感染。

Infection of ESCs by the murine leukaemia viruses results in the normal establishment of integrated proviral DNA, but this DNA is then transcriptionally silenced, preventing further viral spread.

通过鼠类白血病病毒感染ESCs导致整合前病毒DNA的正常建立，但这种DNA而后变为转录沉默，阻止了病毒的进一步蔓延。

However, in the absent of LEF-2, the formation of viral nucleocapsid derived from lef-2 ko bacmid appeared to be normal under transmission electron microscope, suggesting that the life cycle of lef-2 ko bacmid could proceed through DNA replication to viral pakckaging unimpededly.

虽然病毒之复制受到lef-2基因缺失的影响，但由於病毒之构造蛋白仍可表现，如鞘蛋白(VP39)等，因此经由穿透式电子显微镜观察，可知缺失lef-2之病毒仍可有正常的核酸蛋白鞘形成。

Compared with the situation that other defends the virus method,this method has very good hiding amount of information,can take precautions against the executable file computer virus that has already known,ones that can be taken precautions against unknownly too and this kind of computer virus in the future.

分析了该方法的长处和不足，与其它防病毒方法相比，该方法具有很好的信息隐藏量，能防范已知的可执行文件类计算机病毒，也能防范未知的和未来的该类计算机病毒。

To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1. According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.

根据A型流感病毒密码子使用偏嗜性，选取稀有密码子对A/Puerto Rico/8/34(H1N1)病毒NS1基因内部110个氨基酸区域进行密码子同义突变改造，并全基因合成NS基因，利用反向遗传操作技术拯救出含有密码子去优化NS1基因的重组病毒。

Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of culture, cell density can reach 6~7×106/mL, then rabies virus VaG strain was inoculated with a MOI of 0.01. After 24h inoculation, the cultured viruses were harvested continuously for 12 days. The titer of collected virus in the culture medium was about 6.0 to 8.5 logLD50/mL. From these collected viruses, vaccines were prepared through the steps of concentration, inactivation and purification, The quality of these vaccines meets "The Chinese Requirements for Biologics Year 2000".

在5L体积的生物反应器中，加入含10g/L微载体的199培养基，接种Vero细胞至细胞浓度达到1×105/mL，培养7d后细胞可生长至6~7×106/mL，然后以感染复数为0.01接种狂犬病毒VaG株，接毒后24h开始收获，连续收获12d左右，收获的病毒滴度范围在6.0~8.5logLD50/mL，收获的病毒原液经浓缩、灭活和纯化等步骤制备成疫苗，各项质量指标均达到《中国生物制品规程》2000年版要求。

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化，病毒最终滴度达到109 PFU/ml；3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长，这与本课题组在在体动物实验中发现的现象一致。

The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

为了克隆禽腺联病毒(Avian adeno-associated virus， AAAV)全基因组用于构建基因转移载体研究，以鸡胚致死孤儿病毒作为辅助病毒与AAAV共接种SPF鸡胚进行AAAV的增殖，将AAAV约4.7 kb双链基因组DNA与pCR2.1载体连接，构建了含AAAV全基因组的重组质粒pAAAV并进行了测序。

Methods By using Vero cell as the host cells and with acyclovir as the positive control, anti-HSV-1 activity of the extract was observed and determined with cytopathic effect assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects: virucidal assay, attachment assay and penetration assay.

以Vero细胞为宿主细胞，阿昔洛韦为阳性对照药物，通过观察细胞病变效应与空斑减数实验测定川楝子提取物抗HSV-1活性，计算其IC50与治疗指数，并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响三个方面初探川楝子提取物抗HSV-1活性的机理。

The percentage of active transmission of small brown plant hopper varied with the infection stage of RBSDV in maize cell while the nymph acquiring feeding. There were 2.93% and 7.83% of viruliferous rate resp

用灰飞虱无毒若虫在细胞内病毒基质出现和病毒增殖期饲毒的，到成虫时分别有2.93%和7.83%个体传毒率；在细胞内病毒成串分布于管状结构和晶格状聚集排列期饲毒的，到成虫时均不能传毒。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了