病毒

The SARS virus origin which obviously differs from the theory of out space origin and the theory of wildlife origin was theoretically discussed based on principles of pollution evolution ecology,pathogenesis and infectant characteristics of SARS,and some relevant experimental evidence. It emphasizes on the fact that increasingly combined pollution in China provides a substantial basis for virus evolution.

针对当前广为流传的SARS病毒"太空起源说"和"野生动物来源说"两种理论，本文根据污染进化生态学原理和SARS病毒发病传染特征，以有关实验结果为科学依据，阐述了SARS病毒的起源问题，指出：我国当前环境日益严重的复合污染为SARS致病病毒的产生提供了适宜的外部生态条件和物质基础，SARS致病病毒可能起源于多个病毒的基因重组或融合，更可能是一种来自于感冒病毒进化而形成的超级感冒病毒。

It includes four parts: 1 Part I, it introduces the basic knowledge of computer viruses such as computer virus definition, history and important viruses events, features, behavior phenomena, difference with computer software and hardware troubles, damage behavior and capability, classification, naming, future trend, etc; 2 Part II, it introduces the corresponding knowledge of computer systems related to computer viruses such as the composing of software and hardware of the computer system, storage media and its working mechanism, interrupt technologies,.com/.exe/.pe file formats and their working mechanism, etc; 3 Part III, it introduces computer virus mechanism and theory such as computer virus structure, work flow, working mechanism of key modules, typical technologies used to design computer viruses including the corresponding traditional technologies (interrupt filching, memory resident, etc.), the corresponding new routine technologies (self-encrypting, Mutation Engine, etc.), and the corresponding new technologies used by some prevalence computer viruses such as macro viruses, e-mail viruses, worm, hacker, Trojan, mobile phone viruses, the working mechanism analysis of some kinds of typical and prevalence computer viruses such as file-type viruses, macro viruses, e-mail viruses, worm viruses, hacker, Trojan, mobile phone viruses, the analysis of some typical and prevalence computer viruses examples including BALL viruses, WORD macro viruses, WantJob viruses, Code Red viruses, BO Trojan, etc; 4 Part IV, it introduces the defense and killing technologies of computer viruses such as the aim and criterion of computer viruses defense and killing, prevention methods and corresponding technologies, detection technologies (comparison method, character code scanning method, behavior inspecting method, analysis method, etc.), manual and automatic killing technologies, immunity technologies such as IBM digital immunity system, new anti-viruses technology trends (real-time anti-viruses technologies, 32 kernel technologies, active kernel technologies, etc.), some typical virus defense and killing softwares (Symantec AntiVirus product, PC-Cillin AntiVirus product, etc.), the defense and killing method analysis of some kinds of typical and prevalence computer viruses (file-type viruses, macro viruses, worm viruses, hacker, etc.), for example, firewall and intrusion detection technologies for anti-hacker, the defense and killing of some typical and prevalence computer viruses examples including WORD macro viruses, Code Red viruses, BO Trojan, etc.

课程内容具体包括四大部分：1）第一部分，介绍计算机病毒基本知识，包括：计算机病毒定义、病毒发展史及重大事件、病毒特点、病毒表现现象及与软硬件故障的区别、病毒破坏行为及危害性、病毒的传播途径及媒介、病毒分类、病毒的命名及计算机病毒技术发展趋势等内容；2）第二部分，介绍与计算机病毒有关的计算机系统相关知识，包括：计算机系统软硬件组成、存储介质结构及工作原理、计算机系统引导机理及流程、中断技术、。com/。exe/。pe等文件格式及工作机理等内容；3）第三部分，讲解计算机病毒机理，包括：计算机病毒的组成结构、病毒工作流程、病毒引导/触发/感染/破坏等模块的工作机理、计算机病毒所采取的编制技术（包括中断窃取/内存驻留等传统编制技术、自加密/隐形/变形机等新的常规编制技术、宏病毒/电子邮件病毒/网络蠕虫/特洛伊木马/黑客/手机病毒等一些新的流行病毒所采取的编制技术等）、一些类型的典型或流行计算机病毒的工作机理分析（包括：引导型病毒、文件型病毒、宏病毒、电子邮件病毒、蠕虫病毒、黑客、特洛伊木马、手机病毒等）、一些典型或流行的计算机病毒实例剖析（包括：小球病毒、WORD宏病毒、求职信病毒、红色代码病毒、冰河木马等）等内容；4）第四部分，讲解计算机病毒防治技术，包括：计算机病毒防治目的、病毒防治策略及规范、病毒在管理和技术上的预防措施、病毒检查技术（包括：比较法/病毒特征码扫描法/行为监测法/虚拟执行法/分析法等）、手工和自动病毒清杀技术、病毒免疫技术（包括：针对某种一次性感染病毒的基于病毒标签的免疫方法/基于自我完整性检查的计算机病毒免疫方法/IBM的数字免疫系统等）、反病毒技术的新发展（包括：实时反病毒技术/32位内核技术/主动内核技术/以毒攻毒技术等）、诺顿/趋势/金山等公司的病毒防治软件产品、一些类型的典型或流行的计算机病毒（包括：引导型病毒、文件型病毒、宏病毒、蠕虫病毒、电子邮件病毒、手机病毒、黑客、特洛伊木马）的防治措施（其中，也包括介绍面向防范黑客攻击的防火墙、入侵检测技术）、一些典型或流行的计算机病毒防治实例剖析（包括：WORD宏病毒、红色代码病毒、冰河木马v1.1/v2.2等）、多层次病毒防护体系等内容。

Viral infection of the host cell may be switched on by the specific and stable combination of one or several viral envelope protein with one or several glycoprotein receptors available on the cell membrane, which is followed by viral pinocytosis or viral fusion with cell membrance, viral deenvelope, viral particle movement from cytoplasm towards nucleus and the viral gene passage.

病毒感染细胞可能是通过一个或几个病毒包膜蛋白与一个或多个细胞膜上的糖蛋白受体特异稳定地结合而启动，通过病毒胞饮作用或者病毒与胞膜融合、病毒脱包膜、病毒粒子从胞浆到胞核的运动和病毒基因通过核膜的传代将会发生；但参与这些动力过程相关的病毒、细胞结构和生化因子仍不清楚。

The methods for determination of the complete genome sequences of potyviruses, carlaviruses and potexviruses were also established. The complete genome sequences of 13 viruses(20 isolates) were determined while 10 of them were the first report in the world.Five of them including Lily symptomless virus (NC_005138), Lily mottle virus(NC_005288),Onion yellow dwarf virus (NC_005029), Lily virus X (NC_ 007192) and Shallot yellow stripe virus(NC_007433) were selected as the standard sequences of those viruses by National centery of Biotechnology Information, USA.

建立了马铃薯Y病毒属、麝香石竹潜隐病毒属和马铃薯X病毒属成员的基因组全序列扩增技术，完成了13种不同植物病毒近20个分离物的基因组全序列测定，其中10个病毒的基因组全序列为国际首次报道，百合无症病毒(NC_005138)、百合斑驳病毒(NC_005288)、洋葱黄矮病毒(NC_005029)、百合X病毒(NC_ 007192)和葱黄条病毒(NC_007433)等5条序列被美国国家生物技术信息中心列为相关病毒标准序列的有。

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

According to the harm degree after virus erupts and consequence, the poison of 10 a serious illness 2007 is ordinal for: U dish virus of verminosis poison, ARP, net swims virus of great robber virus, MSN sexy album, ANI virus, machine dog virus, acting trojan virus, AV killer virus, Real script virus and virus of panda burn joss sticks.

根据病毒爆发后的危害程度和影响力，2007年的十大病毒依次为：U盘寄生虫病毒、ARP病毒、网游大盗病毒、MSN性感相册病毒、ANI病毒、机器狗病毒、代理木马病毒、AV杀手病毒、Real脚本病毒和熊猫烧香病毒。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

Can detect and remove rootkits, mass-mailing worms, e-mail viruses, peer-to-peer viruses, Internet worms, file viruses, Trojans, stealth viruses, polymorphic viruses, bodiless viruses, macro viruses, MS Office viruses, script viruses, spyware, spybots, password stealers, keyloggers, paid dialers, adware, riskware, hacktools, backdoors, joke programs, malicious scripts and most other malware.

可以检测和删除的rootkit ，邮件群发蠕虫，电子邮件病毒，点对点病毒，互联网蠕虫病毒，文件病毒，木马，隐形病毒，多态病毒，脱胎病毒，宏病毒，病毒的MS Office ，脚本病毒，间谍软件， spybots ，密码盗取者，键盘记录程序，支付拨号，广告软件， riskware ， hacktools ，后门，玩笑程序，恶意脚本和其他大多数恶意软件。

Abstract］ objective for the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.methods aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with ph 4, human immunoglobulin and human hepatitis b immunoglobulin for intravenous injection with ph 4/pasteurization,treatment on human fibrinogen and human coagulation factor ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.the mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.results human albumin pasteurization inactivated vsv and sindbis virus,hrig low ph inactivated vsv and sindbis virus,hrig low ph inactivated hiv virus,ivig low ph/pasteurization inactivated hiv virus,ivig low ph inactivated hiv virus,hbig low ph/pasteurization inactivated vsv,sindbis and polio virus.conclusion suggestion about improvement on unreasonable parameter and standard of virus inactivation is made,cultivate target virus directly from final product by appropriate cell or method to monitoring viral safety of blood plasma product is advanced.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低ph常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低ph/巴氏消毒法；人纤维蛋白原、人凝血因子ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。结果人血白蛋白巴氏灭活vsv与sindbis病毒，hrig低ph灭活vsv和sindbis病毒，hrig低ph灭活hiv病毒，ivig低ph/巴氏灭活hiv病毒，ivig低ph灭活hiv病毒，hbig低ph/巴氏灭活vsv、sindbis与polio病毒。结论建议改进病毒灭活验证标准与不合理的灭活参数；采用终产品样品通过合适细胞或方法直接培养目标病毒来有效监测血液制品的病毒安全性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。