病原菌

It was found that the fungus 1403 resembles pathogenic F. verticillioides (teleomophy Gibberella moniliforme) in the production of false head or chains and abundant microconidia on the aerial mycelium, but different in the occasional formation of polyphialides with relatively long as well as short monophialides, in its typical coiled hyphae and mycelia fusion.

基于1403在形态学方面具有以下特征：产小型分生孢子的分生孢子梗较长，小型分生孢子呈椭圆形，大型分生孢子线形或略微弯曲，本文将它与有类似形态特征的镰刀菌进行了比较，发现1403与植物病原菌Fusarium verticillioides（有性态为Gibberella moniliforme）表面上很相似，气生菌丝均可产生大量假头状或链状的小型分生孢子，但深入的研究却发现1403与F。

By means of mycelium growth rate, the acetone extracts of fermentation products of 39 strain were tested against Botrytis cintrea,Gibberella zeae, Phytophthora capsic, Glomerella cingulata, Bipolaris maydis, Alternaria alternata at the concentration of 500 u g/ml. The results showed that the acetone extract of 1L, 2B and 5J strains fermentation products had a certain degree fungicidical activity.

以番茄灰霉病菌、小麦赤霉病菌、苹果炭疽病菌、玉米小斑病菌和辣椒疫霉病菌为供试菌种，在500μg／ml的浓度下，采用抑制菌丝生长速率法对39株内生真菌发酵产物进行了杀菌活性筛选，发现1L、2B和5J 3菌株的发酵产物丙酮粗提物均对供试病原菌有不同程度的杀菌活性。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌，在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛，得到6株对青枯劳尔氏菌有较强拮抗作用的细菌，通过摇瓶发酵后利用发酵液，采用改良的琼脂孔扩散实验复筛，获得一株具有强拮抗作用的菌株，命名为Q6号。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

For Streptococcus penumoniae、Hemophilus in0fluenzae、Staphylococcus aureus、Acinetobacter baumannii、Pseudomonas aeruginosa,the sensitivety of Tem-PCR was 100%、41.7 %、11.1%、50.0 %、66.7 % respectively; the specificity was 84.0%、81.7%、98.9%、95.2%、98.9% respectively. Tem-PCR was more sensitive than culture in the detection of Streptococcus penumoniae 36/19.1% vs.

3以细菌培养作为参考标准，Tem-PCR对14种病原菌总的敏感性为51.0%，特异性为68.0%，符合率为58.3%，对肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、鲍曼氏不动杆菌、铜绿假单胞菌的敏感性分别为100%、41.7%、11.1%、50.0%、66.7%，特异性分别为84.0%、81.7%、98.9%、95.2%、98.9%。

It was heterothallic and could not produce oospores in natural gingko-growing regions. But it could produce a large number of chlamydospores in adverse environments,such as low temperature.

该病原菌寄主范围较广，生长温度大于35℃，异宗配合，自然条件下不能产生卵孢子，但在低温等不良环境下形成大量厚垣孢子。

In this study, gaschromatographic analysis was used to identify hydrolytic type of VHH.

在已知的弧菌中，约20种是海水养殖动物的病原菌。

The Escherichia coli is the most common pathogenic bacilli both on the medicineand the clinical veterinarian, the pathogenic can cause young pig"s yellow diarrheaand white diarrhea,and pig"s hydropsy, and so on.

大肠杆菌是医学和兽医临床上最常见的一种病原菌，其致病性株可引起仔猪黄痢、白痢、猪水肿等病。

The spores of hyperparasitic Streptomyces strains PR and F46 were irradiated with UV light to improve the control effects against plant diseases.

本研究以重寄生链霉菌PR 和F46 为出发菌株，以靶标病原菌为指示菌，以紫外线为诱变因子，对它们进行了诱变改良研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。