疫苗原

Therefore, to analyze difference of immunogenicity among NDV epizootic isolates and vaccine strains in details and to establish methods to check immune and epidemic situation,we expressed F protein of strain F_(48)E_9 and strain La Sota respectively.

为了具体分析流行毒株和疫苗毒株之间的免疫原性差异情况和建立适用于检测ND免疫和流行情况的方法，我们又分别表达了弱毒疫苗株La Sota和标准强毒株F_(48)E_9的F蛋白。

As showed above, we could draw the conclusion that H3 viruses could cause outbreaks or local epidemics in Shanghai for the immune of population to the new emerging antigenic drift was still unknown though there was strong immune barrier to the previous vaccine strain, H1 viruses also may caused epidemics as the population had a weak immune protection to the previous reference strains A/New Caledonia/20/99-like and part of the recent isolates were distingwishing from them and continued to evolute on the new reference strain, and H5N1 HPAI activities has expanded its geographical range thus increasing the size of the population at risk as the continuing emergence of human cases which give the virus an opportunity to evolve towards a fully transmissible pandemic strain. We should keep eyes on the human cases of avian influenza virus an its variation for the H5 isolates in Shanghai also has the possibility to infect human directly and the infection of H5, H9 viruses had previously occurred in the population especially in those contacting with animals.

综合上述分析结果，我们认为上海地区人群虽然对原有的H3亚型参考病毒株有较高的免疫保护，但H3亚型病毒HA已出现新的抗原漂移，人群现在对新变异株的保护水平尚未知，有可能在未来引起局限性暴发甚至地方性流行；人群对H1亚型流感病毒免疫保护水平低，同时H1亚型流感病毒株部分与原推荐疫苗株抗原性差别较大，并在现推荐疫苗株基础上继续变异，需警惕发生流行的可能性；H5N1亚型高致病性禽流感地域范围扩大，人间病例不断发生，存在演变成大流行株的威胁，上海市H5亚型禽流感病毒株存在跨宿主屏障感染人类的可能性，而且已发现人群尤其禽、畜类动物接触人群中存在H5、H9亚型流感病毒的既往感染，应警惕禽流感病毒的变异和人间病例的发生。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.

对 2 0日龄 SPF猪和 2 0日龄猪繁殖与呼吸综合征血清阳性猪，人工感染猪繁殖与呼吸综合征病毒北京分离株( BJ-4 )后 4 8h接种猪瘟疫苗，利用 ELISA方法检测仔猪针对 PRRSV和猪瘟疫苗的体液免疫，利用 MTS法检测仔猪外周血单核细胞对有丝分裂原 Con A的刺激反应。

Company to return to Taiwan Sheng and Huazhong Agricultural University for technical support, with a number of highly qualified to doctorate or master's degrees as the backbone of science and technology development and technical services team, has developed a Apramycin new Dasheng, Yakult element go from, Yinqiaosan, bacteria and many other high-tech Fukang animal health products; at the Chinese Academy of Engineering, led by Professor Chen Huanchun has developed Pig Pseudorabies Inactivated vaccines, gene deletion pseudorabies vaccine, Porcine Pleuropneumonia hole inactivated vaccine agent, porcine reproductive and respiratory syndrome virus vaccine and inactivated vaccine, pig cells attenuated Japanese encephalitis vaccine more than 20 new vaccines and diagnostic kits.

公司以台湾回盛和华中农业大学为技术支撑，拥有一批高素质的以博士、硕士为骨干的科技开发和技术服务队伍，先后开发了安普新、大盛、益力素、去从、银翘散、菌福康等多种高科技动物保健产品；在中国工程院院士陈焕春教授带领下，先后开发了猪伪狂犬灭活疫苗、猪伪狂犬病基因缺失活疫苗、猪传染性胸膜肺炎油孔剂灭活苗、猪繁殖与呼吸综合征弱毒疫苗和灭活疫苗、猪乙型脑炎原代细胞弱毒疫苗等20余种新型疫苗和诊断试剂盒。

Abstract］ Objective:To purify and characterize the major antigenic components of sand swimming crab,and provide theoretical evidences for the research of standardization of allergenic vaccine.Methods:The crude extract of Linnaeus was prepared using classical method, and electrophoresis of SDSPAGE was used to separate the proteins of each group.

目的：对梭子蟹变应原进行分离，鉴定其主要及次要变应原，采用蛋白纯化技术获取梭子蟹天然的主要变应原并进行鉴定，为标准化变应原疫苗的研制提供理论依据。

Objective: To investigate the antigenic components of Amaranthus spinosus pollen, and to lay theoretical base for the purification of Amaranthus spinosus pollen allergens and the standardization of allergenic vaccine.

目的：分析刺苋花粉的抗原成分以及刺苋花粉特异性变应原组分，为刺苋花粉变应原的纯化和标准化变应原疫苗的研制提供理论依据。

Humulus scandens grass pollen major allergen DNA vaccine pcDNA3.1-LC2 has been successfully constructed. It can be expressed in mice, produces humulus scandens grass pollen allergen specific antibody and has a good immunogenicity.

成功的构建了葎草花粉主要变应原核酸疫苗pcDNA3.1-LC2，pcDNA3.1-LC2可在小鼠体内表达，并能产生葎草花粉变应原特异性抗体，具有良好的免疫原性。

Healthy rabbits were inoculated each with vaccines of the recombinant Pilin and the recombinant fused Pilinn-IL2 protein at 250ug per dose to potentiate the immunogenicity. The D. nodosus Pilin vaccine and the killed D. nodosus whole cell vaccine were also inoculated into rabbits as controls in order to compare with the above two recombinant vaccines.

用纤毛蛋白基因工程疫苗、Pilin-IL-2融合基因工程疫苗分别免疫健康家兔，初步进行了纤毛蛋白疫苗和Pilin-IL-2融合基因工程疫苗的免疫原性试验，并同时用节瘤拟杆菌菌体灭活疫苗及菌体纤毛蛋白疫苗免疫家兔，进行了免疫原性比较试验。

Rabic vaccine is one of the earlest and the most successful vaccine used for human. It's development went through several stages: animal brain tissue live vaccine, inactivated vaccine, prima cells culture inactiveted vaccine and cells cullture purified vaccine. Rabic vaccine plays a positive role in that prevent and control rabic epidemic.

人用狂犬病疫苗是世界上最早研制成功和使用的疫苗之一，经历了用动物脑组织制备的活疫苗、灭活疫苗、原代细胞培养灭活疫苗和细胞培养精制纯化疫苗等几个阶段，它为人类防治和控制狂犬病的传播起了积极的作用。

The result shows: 3bring content to be able to detect from inside vaccinal sample to the pollution that supports former put oneself in another's position to pcr, 3 pairs of check that make thin

结果显示：3对pcr引物都可以从疫苗样品中检测到支原体的污染，3对引物的检出率分别为20%、17.1%和14.3%，说明猪瘟活疫苗中的支原体污染为多种支原体的混合污染，污染源较为复杂。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。