疫苗

Objective To explore the feasibility of heparinase vaccine in active immunity for gastric cancer.

目的研究肝素酶基因修饰的树突状细胞疫苗对胃癌细胞的免疫效应，探讨肝素酶疫苗在胃癌主动免疫治疗中的可行性。

GeoVax chose Althea as the contract manufacturer for its DNA vaccine based on its extensive experience in the field of DNA vaccine manufacture and its regulatory compliance history.

GeoVax 之所以选择Althea作为DNA疫苗的合作伙伴，是因为后者在DNA疫苗生产领域有着丰富的经验和严格遵守法规的历史。

Our research was motivated by the fact that the current anthrax vaccine has significant limitations and there is a great need for a better one.

""我们的研究是出于一个事实，即目前的炭疽疫苗具有很大的局限性，新疫苗有大量的需求。

"Our research was motivated by the fact that the current anthrax vaccine has significant limitations and there is a great need for a better one."

我们的研究基于事实，就是目前的炭疽疫苗具有局限性，我们需要一种更好的疫苗。

The results suggest that these six vaccines may be used in some emergency condition with proper order and immune period, but there may remain some risks in the multiple vaccines administration especially these with plague and anthrax vaccine.

结论出血热、霍乱、痢疾、钩体疫苗等疫苗在采用恰当的接种顺序和接种间隔的情况下可以联合使用，但是可能存在一定的风险，尤其在此基础上加入炭疽和鼠疫疫苗。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

This is wrong, the state provided the vaccine only a few, such as the influenza vaccine is not in his provisions, but the benefits of influenza vaccine we all know, so as long as it is to the regular injection of epidemic prevention station or hospital, and doctors say they can fight , I would like to have no problem.

这话不对，国家规定的疫苗只有那么几种，比如流感疫苗就不在他规定内，但是流感疫苗的好处大家都知道，所以只要是去正规的防疫站或医院注射，并且是医生说可以打的，我想都没有什么问题。

Our research work investigated the possibility of applying the vaccine to gene therapy of CEA positive tumors and set the foundation for further study of its clinical application.

为解决上述问题、提高基因治疗的安全性，本研究采用细胞移植免疫用隔离工具-微胶囊包裹转染部分CEA基因的人成纤维样骨髓基质细胞HFCL，制备出CEA微囊化转基因细胞疫苗，并进行了疫苗在小鼠体内的表达及其免疫学性质的研究，探讨该疫苗应用于CEA阳性肿瘤治疗的可能性，并为进一步的临床应用研究奠定了基础。

In particular, the technology of the use of transgenic plants to produce human or animal therapeutic vaccines receives increasing atten-tion. Expressing vaccine candidates in vegetables and fruits open up a new avenue for producing oral/edible vaccines. Transgenic plant vaccine disquisitions exhibit a tempting latent exploiting foreground.

尤其是利用转基因植物技术生产植物疫苗的研究受到了广泛的关注，在转基因植物的可食用部位表达抗原生产人或动物治疗用重组蛋白和疫苗的技术为可食性疫苗的研制开辟了新途径，展现了诱人的开发前景。

The findings showed that mothers' knowledge about the primary course of DPT was low (33% correct). The primary source of knowledge was from healthcare providers. Mothers' educational level was statistically associated with know ledge of the DPT vaccine.

研究结果显示：生育第一胎母亲之第一剂三合一疫苗的知识偏低（答对率33.1%）；主要知识来源为医院的医护人员；母亲的教育程度与疫苗之知识的了解呈显著相关，即较高的教育程度有较高的疫苗知识。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。