疫苗

Major suppliers of the Company's products mainly include: genetic engineering products, a variety of blood products, various types of vaccine: If chicken pox vaccine, rubella vaccine, influenza vaccine, Ma gills diad vaccine, hepatitis A vaccine, rabies vaccine, such as more than 20 varieties.

本公司主要供应的产品主要有：基因工程产品、各种血液制品、各类预防疫苗：如水痘疫苗、风疹疫苗、流感疫苗、麻腮二联疫苗、甲肝疫苗、狂犬疫苗等二十多个品种。

Vaccines for BCG, Sabin, measles vaccine, German measles and chicken poxare all active vaccines. Three-in-one vaccines(anti-diphtheria vaccine,pertusis vaccine and 破伤风), and vaccines for encephalitis B, hepatitis B

卡介苗、口服性小儿麻痹疫苗、麻疹、德国麻疹疫苗、和水痘疫苗都是属於活性灭毒的疫苗，而白喉、百日咳、破伤风、日本脑炎、B型肝炎和流行性感冒疫苗则属於非活性疫苗。

Onlythe Yale University medical school, the nursing institute and assistant doctor the project requested the student to vaccinate specially parotitis vaccine, smallpox vaccine, the second grade hepatitis vaccine, tetanus vaccine, the infantile paralysis vaccine, hepatitis A vaccine (or hepatitisA and hepatitis B combination vaccine), typhus vaccine, yellow fever vaccine and the Japanese second grade encephalitis vaccine, as well as provides the hepatitis B blood to examine list and the tuberculin check list (tuberculin exceed the allowed figure also needs to provide chest X light check list).

只有耶鲁大学医学院、护理学院和助理医师项目特别要求学生接种过腮腺炎疫苗、天花疫苗、乙型肝炎疫苗、破伤风疫苗、小儿麻痹症疫苗、甲肝疫苗、伤寒疫苗、黄热病疫苗和日本乙型脑炎疫苗，以及提供乙肝血检单和结核菌素检查单（结核菌素超标者还需要提供胸部X光检查单）。

Currently,this vaccine has been studied for the prevetion of leishmaniasis,malaria,schistosomiasis and other parasitic diseases.

核酸疫苗包括DNA疫苗和RNA疫苗，目前研究较多的是DNA疫苗，由于它不需要任何化学载体，所以又称为裸疫苗。

The Shanghai MMR vaccine is as safe as the measles vaccine, mump vaccine, rubella vaccine and imported MMR vaccine which have been used.

沪MMR疫苗具有与目前使用的麻疹疫苗、腮腺炎疫苗、风疹疫苗及进口MMR疫苗相同的免疫安全性。

The protective efficacy of the fusion DNA vaccines was surpass the polyvalent DNA vaccines and equivalent to that of the BCG vaccine, suggesting that fusion DNA vaccines represent a promising approach for the prevention of BT. So our experiment may be useful for the development of Bovis tuberculosis vaccine.

从攻毒保护效果来看，融合DNA疫苗组的保护效果明显优于多价DNA疫苗，达到了BCG疫苗的免疫保护水平，表明本研究制备的融合DNA疫苗具有良好的用前景，从而为牛结核病新型疫苗的研究奠定了基础。

This paper was to observe the revaccination effect of domestic yeast-derived recombinant hepatitis B vaccine to children who were born to HBsAg and HBeAg negative mothers and had been immunized with full course of blood-derived hepatitis B vaccine 4 years ago at their birth.

为了观察用国产重组酵母乙型肝炎疫苗，对已经血源乙肝疫苗初免的儿童再免疫的效果，以乙肝表面抗原和乙肝e抗原阴性母亲所生的、出生时全程接种血源乙肝疫苗后已4年的儿童为对象，用5μg重组酵母乙肝疫苗进行再免疫，同时用10μg血源乙肝疫苗再免疫作对照，观察2种疫苗再免疫后1、12、24个月乙肝表面抗体反应。

Why inoculation point stressed Doctor candy vaccine polio after taking half an hour not to drink hot water or breast-feeding was mainly due to candy are live attenuated vaccines, if sent to serve with warm water will inevitably lead to failure of inactivated vaccine, which is why We have seen many of the Vaccine Inoculation point out from the refrigerator and placed on ice floe, and this is what we usually have to go through the vaccine cold chain system, the only way to ensure the vaccine will not be denatured failure.

为什么接种点医生强调服食脊灰糖丸疫苗后半小时内不要喝热开水或者喂奶，主要是由于糖丸是减毒活疫苗，如果用温水送服必然导致疫苗灭活失效，这就是为什么我们见到许多接种点的疫苗要从冰箱拿出来，并且放在冰排，这就是我们通常所说的疫苗要经过冷链系统，只有这样才能保证疫苗不会变性失效。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The causative agent of the disease is an Corona virus, infectious bronchitis virus IBV）, classified as Corona .Present vaccines for IB are all modified live viruses derived either by sequential passage embrocated chicken eggs. As Immunogenicity of IBV is usually correlated with its virulence, almost all modified live IB vaccines remained insufficient attenuation and have shown a variety of side effects including spread of vaccine virus to no vaccinates, occurrence of long term "carrier" birds, and increasing virulence during in vivo passage.

目前国内外预防本病所用的疫苗大多数是弱毒疫苗，但现用弱毒疫苗都存在以下缺点：（1）与IBV强毒一样，其弱毒疫苗株也能引起潜伏感染，潜伏感染的鸡将终生带毒；（2）疫苗病毒可以由接种鸡传染给非接种鸡，在鸡群之间传播过程中毒力会返强，从而成为新的感染源；（3）传染性支气管炎病毒极易发生变异，且易成为病毒重组的供体和受体，给使用IB传统疫苗预防和根除IB带来极大的困难。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了