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When it comes to pandemics, there is no simple correlation between disease and dysfunctional states.

当大范围的疫病暴发的时候,疾病的流行与政府的机能失调之间的关系也随之变的复杂。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

The experimental results showed that the crude toxin of Phytophthora capsici could inhibit the germination of seeds, the growth of seedlings, and the growth of pepper radicel and the callus of pepper extremely.

结果表明,粗毒素能使辣椒叶片产生与疫病病原菌侵染类似的水渍状褐腐斑,抑制辣椒种子的发芽率,抑制辣椒幼苗和胚根的生长,抑制辣椒愈伤组织生长,并且粗毒素的这些抑制作用随着其浓度的升高而变强,不同品种所得出的实验结果一致。

Such as: cotton wilt, Verticillium wilt, damping-off, wheat, corn, stiff Miao, Miao Huang, yellow leaves, the yellow leaves of fruit trees, anthrax, early defoliation disease, melons, vegetables Peronospora class , leaf molds, spreading litter, Pseudomonas, keratophyre, withered, anthrax, the disease, black rot, soft rot, peanuts, legumes, such as stem blight significant effect.

棉花的枯萎病、黄萎病、立枯病,小麦、玉米的僵苗、黄苗、黄叶、烂秧,果树的黄叶、炭疽病、早期落叶病,瓜菜类的霜霉、叶霉、蔓枯、青枯、角斑、枯萎、炭疽、疫病、黑腐、软腐,花生、豆类的死苗、茎枯病等有显著的防治效果。

Using 11 primers filtrated from total 53 primers, ISSR amplification was conducted in 220 individuals from eleven Cryphonectria parasitica subpopulations which were from four countries, and 174 polymorphic loci were detected from total 177 loci. The percentage of polymorphic loci of the total populations was 98.31%, while that at a subpopulation level averaged 20.43 %. Shannon's Information index and Nei's gene diversity of the fungus at the total populations level was 0.1769 and 0.7386, respectively.

自53个ISSR引物中筛选出11个引物,对以上四个群体的栗疫病菌11个子群体的220个菌株进行扩增,共检测到177个位点,其中多态位点为174个,多态位点百分率为98.31%。11个栗疫病菌子群体的P平均值为20.43%;群体水平的Shannon信息指数和Nei指数分别为0.1769和0.7386;各个子群体I的平均值为0.0943,h的平均值为0.0614。

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