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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

It is reported the control of bacterial leaf blight with 7 kinds of bactericides on the field in Xishuangbanna.

报告7种杀菌剂在西双版纳防治红掌细菌性叶疫病的田间防治效果,其中土霉素100倍液防效最好,达71.1

South American leaf blight,caused by Microcyclus ulei,is one of the most serious disease of Para rubber in America,and the pathogen is an important quarantine pest of the Asia-pacific region.

橡胶南美叶疫病菌在美洲地区引起橡胶树严重的落叶病害,是亚太地区国家重要的检疫性有害生物。

The varieties resistant variety of diseases, such as high resistance to virus diseases and bacterial wilt, early blight resistant, gray mold and leaf mold resistance, low temperature, low light, setting a high rate, non-deformed fruits and empty fruit, the results of a single inflorescence 15 ~ 35, merchandise fruit rate of more than 98% for tomato production in Hebei Province has provided a fine new varieties.

品种抗多种疾病,如高抗病毒病和青枯病,抗早疫病,灰霉病和叶霉病性,低温,弱光,设置率高,不变形的水果和空果,单一花序结果1535,商品的98%以上番茄果实河北省产出率提供了一个优良新品种。

It is by traditional Chinese medicine in beast it is serial in pharmaceutical for development and employing being of products in particle in in veterinarian theoretical foundation, Combine whom modern traditional Chinese medicine develop use mainly treatment , poultry of epidemic disease for , innovation its order , lie in series of products this belong to pure traditional Chinese medicine particle, not so incomplete as medicines, curative effect is precise ; Employ modern detection means to determine the effective composition, improve the production technology, there is no quantitative current situation to makeover traditional Chinese medicine, it is controllable to reach quality , and produce industrialization by animal remedy GMP management.

项目简介:兽用中药颗粒剂系列产品的开发及应用是在中兽医理论基础,结合现代中药开发的主要用于禽类疫病的治疗,其创新点在于该系列产品属纯中药颗粒,无药残,疗效确切;并应用现代检测手段测定有效成分,改进生产工艺,彻底改变中药无定量的现状,达到质量可控,并以兽药GMP管理进行产业化生产。

A blight may be but a local infection easily controlled; on the other hand, it can attack particular plants in a whole region or nation.

一种疫病也许只在局部范围内流行,容易控制。另一方面,它也可能在整个地区或整个国家为害某些作物。

The public health systems were improved step by step. Network report system of epidemic situation was brought into execution in all the medical and health units above town-level so as to control such diseases as measles, lyssa, bird flu and pig streptococcus effectively.

公共卫生体系逐步完善,全区镇以上医疗卫生单位均实现重大疫情网络直报,麻疹、狂犬病、禽流感、猪链球菌等疫病得到有效防控,儿童计划免疫接种率达95%以上,甲乙类传染病总发病率589/10万。

Milch cow industry is one of important part of dairy industry, including breeding milch cow ,raising milch cow , preventing and cure of the epidemic disease and milk production.

奶牛业是奶业系统的一个重要组成部分,主要包括奶牛培育、奶牛饲养、疫病防治和原奶生产四个环节。

Each kind of date age's muscovy duck may also infect the morbidity, epidemic disease propagation velocity is quick, one week may infect the entire group, the disease incidence rate and the mortality rate may reach as high as 80%.

各种日龄的番鸭也可感染发病,疫病传播速度快,一周可传染全群,发病率和死亡率可高达80%。

Objective:To study the effect of newcastle disease virus on the human lines of gastric cancer cells.

目的:研究新城疫病毒对人胃癌细胞株的作用。

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