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Key Laboratory of Zoosis Disease Control and Prevention of Guangdong Province/Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture/College of Veterinary Medicine, South China Agricultural University,Guangzhou 510642,China

华南农业大学兽医学院农业部动物疫病防控重点开放实验室广东省动物源性人畜共患病预防与控制重点实验室,广东广州510642

In this study, the DNA fragments coding for amino acids 133~158 of VP1and 20~34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133~158-20~34-133~158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133~158位氨基酸残基确是一重要B细胞中和抗原表位的基础上,依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列,采用真核偏爱密码子化学合成了VP1中编码133~158位氨基酸及VP4中编码20~34位氨基酸这两个抗原表位基因,将其组成133~158-20~34-133~158串联结构,在其5′端加上鼠Igκ链信号肽序列,在翻译调节区加上增强表达的Kozak序列,同时在133~158-20~34-133~158串联结构3'端终止密码子下游加上CpG免疫刺激序列,将这些片段连接后,克隆到真核表达载体pHook-2上,构建成功了DNA疫苗重组表达载体pAS1-E。

Local officials must feel that this surveillance and response programme serves their communities, and that there is a sense of joint ownership, said Eman Sulaeman, a PDS team leader in West Java's Bandung Livestock Services.

地方官员必须认识到,这种监测和应对计划是为其社区服务,要有共同的主人翁意识,西爪哇的万隆家畜服务局参与性疫病监测工作组组长Eman Sulaeman说。

Whenever fear assails you, whenever your children are exposed to danger or contagion, realize that "He shall give His angels charge over them, to lead them in all their ways."

无论何时恐惧来袭,无论你的孩子暴露在怎样的危险和疫病的环境之中,只要你意识到&上帝会派他的天使们来接管这些孩子,带领着他们各自前行。&

Article 21 In the case of an animal epidemic of Class I, the animal husbandry and veterinary administrative department under the local people's government at or above the county level shall dispatch its personnel to the scene forthwith to delimit the epidemic point, the epidemic area and the threatened area, collect epidemic materials, investigate into the epidemic source, report in time to the people's government at the same level for a decision of cordoning off the epidemic area, and report the epidemic situation and the related information level by level to the animal husbandry and veterinary administrative department under the State Council.

第二十一条发生一类动物疫病时,当地县级以上地方人民政府畜牧兽医行政管理部门应当立即派人到现场,划定疫点、疫区、受威胁区,采集病料,调查疫源,及时报请同级人民政府决定对疫区实行封锁,将疫情等情况逐级上报国务院畜牧兽医行政管理部门。

It is currently fashionable to consider atopic dermatitis, like other inflammatory dermatoses, as immunologic in pathogenesis ("inside-outside" hypothesis)....

目前现行对遗传过敏性皮炎,就像其他的发炎性皮炎一样,是以免疫病理学的方向来考量(&inside-outside&假说)。

It is currently fashionable to consider atopic dermatitis, like other inflammatory dermatoses, as immunologic in pathogenesis ("inside-outside" hypothesis)....

目前现行对遗传过敏性皮炎,就像其它的发炎性皮炎一样,是以免疫病理学的方向来考量(&inside-outside&假说)。

With gurgling guffaws, the Greater Deamon sends forth his legions with extravagant waves of his arms, extolling the virtues of plague and pestilence, booming words of encouragement or reproach across the battlefield.

伴随着咯咯的粗野大笑,大恶魔用夸张挥舞他的武器方式鼓舞他的军团前行,对疫病优点的高声赞颂,合着鼓励和斥责的隆隆之音穿透战场。

Animal health supervision institutions shall, in light of the needs for animal epidemic prevention and control, assign officially registered veterinaries to stations, ports, airports and other relevant places upon the approval of the local people's governments at or above the county level.

动物卫生监督机构根据动物疫病预防、控制需要,经当地县级以上地方人民政府批准,可以在车站、港口、机场等相关场所派驻官方兽医。

Key Laboratory of Zoonoses of CAAS, Key Laboratory of Veterinary Parasitology of Gansu Province

中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

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