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In this study, genes encoding MAPK and MEK in P. parasitica, an important plant pathogen, were cloned by PCR using degenerate primers, followedby 5'- and 3'-RACE.

在本篇研究中,利用简并式引子对进行聚合酶连锁反应并配合RACE成功从疫病菌中选殖出4个MAPK以及5个MEK基因。

Cryphonectria parasitica, a dangerous forest pathogen, have been prevailing and causing great economic losses in the Chinese chestnut plantations in Kunming area since 2005.This disease mainly infects the trunk and big branches, and the highest mortality was observed during the period from April to June.

板栗疫病作为一种危险性的林木病害,目前已在昆明市的重点板栗种植区普遍发生,并于2005年以来开始形成大面积的暴发危害,相继在一些局部板栗产区造成了较为严重的灾害损失。

The antibiotic 2507 has also satisfactory effect for controlling chinese cabbage downy mildew disease infected by Peronospora parasitica Fr. with efficacy 87.5% in field test.

结果表明,抗生素2507在温室人工接菌情况下,防治黄瓜疫病的效果可达95%以上,田间小区防治小白菜霜霉病的效果达到87.5%。

Using 11 primers filtrated from total 53 primers, ISSR amplification was conducted in 220 individuals from eleven Cryphonectria parasitica subpopulations which were from four countries, and 174 polymorphic loci were detected from total 177 loci. The percentage of polymorphic loci of the total populations was 98.31%, while that at a subpopulation level averaged 20.43 %. Shannon's Information index and Nei's gene diversity of the fungus at the total populations level was 0.1769 and 0.7386, respectively.

自53个ISSR引物中筛选出11个引物,对以上四个群体的栗疫病菌11个子群体的220个菌株进行扩增,共检测到177个位点,其中多态位点为174个,多态位点百分率为98.31%。11个栗疫病菌子群体的P平均值为20.43%;群体水平的Shannon信息指数和Nei指数分别为0.1769和0.7386;各个子群体I的平均值为0.0943,h的平均值为0.0614。

The character of hemagglutination of the virus could be inhibited by the antiserum of Newcastle disease virus.According to the international standard and method of virulence judgement of NDV, we measured the average death time、the minimal lethal dose and the median infective dose(EID50) of chicken embryos and the intracellular inoculation pathopoiesis index of 1-day-old chicks.

参照国际上规定的新城疫病毒毒力判定标准及其方法对分离毒株ZH1、ZH2进行了鸡胚最小致死量和平均死亡时间、鸡胚半数感染量(EID50)以及1日龄鸡脑内接种致病指数测定,结果ZH1、ZH2株的MDT为52h和44 h,EID50为106.4/ 0.1mL和108.64/ 0.1mL,ICPI为1.93和1.975。

A one-step reverse transcription loop-mediated isothermal amplification assay was developed for rapidand sensitive detection of peste des petits ruminants virus.

本研究采用一步法反转录环介导等温扩增技术建立了一种快速、灵敏、高度特异的检测小反刍兽疫病毒的方法。

The inhibitory activity of Echcin to 14 strains of phytopathogenic bacteria was tested using paper plate method.

采用纸碟法测定了Echcin 对5 个属植物病原细菌共14 个菌株的抑制作用,琼脂稀释法测定了Echcin 对12 种植物病原真菌的抑制作用,在此基础上开展了Echcin 对番茄早疫病和稻白叶枯病的温室盆栽试验,同时开展了Echcin 对小麦纹枯病的田间小区试验。

Under controlled conditions in growth chamber, this paper studied the mortality of pepper seedlings caused by Phytophthora capsici.

在生长箱内控制条件下分析测定了土壤温度、水分含量对辣椒疫病死苗率的影响。

Thus, risk of flumorph resistance development in? Phytophthora capsica? appears to be relatively low.

由此可见辣椒疫病菌对氟吗啉的抗性风险可能较低。

Aggregate score repositories may be a blight on the pimply countenance of games journalism, but they may also be able to tell us something about the ...

总计分数存放可能成为疫病对pimply国家进行的游戏新闻,但他们可能还可以告诉我们一些有关。。。

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