疫

Foot and mouth disease virus causes a highly contagious infectious disease foot-and-mouth in cloven-hoofed animals. This disease is widely endemic all over the world and often results in considerable economic losses.

口蹄疫(foot and mouth disease，FMD)是由口蹄疫病毒(foot and mouth disease virus，FMDV)引起偶蹄动物的一种传染病，它严重危害家畜的生产力和畜产品质量，给畜牧业生产和进出口贸易造成较大的经济损失，直接威胁着国际贸易和国家声誉及人类食品卫生，因而受到人们的普遍关注。

Foot and Mouth Disease, a major threat to cloven-hoofed animals such as bovine, swine and sheep, is a highly contagious disease caused by Foot and Mouth Disease Virus. Since it has a high incidence and can easily cause an epidemic outbreak and enormous loss of economy, Office International des Epizooties ranks it in the first in all contagious diseases for domestic animals.

口蹄疫(Foot and mouth disease， FMD)是由口蹄疫病毒引起的主要危害牛、猪、羊等偶蹄动物的烈性传染病，发病率高，传染性强，并能形成国际大流行，国际兽疫局将其排在A类家畜传染病的首位。

Foot-and-mouth disease is a highly contagious disease of cloven-hoofed animals, whose causative agent,foot-and-mouth disease virus,is a member of the Aphthovirus genus within the Picornaviridae.

口蹄疫(Foot-and-mouth disease，FMD)是由口蹄疫病毒(Foot-and-mouth disease virus，FMDV)引起的多种偶蹄动物高度接触性传染病。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The isolate revealed to be able to agglutinate chicken haemocytes with the agglutination reaction to be inhibited by Newcastle disease virus standard antiserum.

该病毒凝集鸡红细胞的作用可被新城疫标准阳性血清所抑制，证明所分离的毒株为新城疫病毒。

Outbreak of measures to be taken: the culling of all poultry and poultry all died, were culled poultry and poultry products in accordance with the relevant provisions of sound processing; poultry droppings, contaminated feed, Bedding , sewage and other sound processing; of contaminated items, transport, equipment, poultry house, the venue strictly thoroughly disinfected, the eradication of foci.

疫点内应采取的措施：扑杀所有的禽只，并对所有病死禽、被扑杀禽及其禽类产品按有关规定进行无害化处理；对禽类排泄物、被污染饲料、垫料、污水等进行无害化处理；对被污染的物品、交通工具、用具、禽舍、场地进行严格彻底消毒，消灭疫源。

The illumination is of no effect on the germination rate of oospores of Phytophthora sojae.30 single-oospore isolates obtained from the test strain of Phytophthora sojae Ps-H2 (Race 1 ) were evaluated using hypocotyl-inoculation method for race phenotype on soybean cultivars. One clone phenotype ( race 1 )was selected randomly for further isolation and virulence identify .

将大豆疫霉菌供试菌株Ps-H_2(生理小种1号)每代挑取30个单卵孢株，进行生理小种鉴定，随机挑选1株1号生理小种的单卵孢株继续进行单卵孢株分离和毒性鉴定，连续两代试验结果表明大豆疫霉菌原始菌株并朱出现毒性分化，表明原始菌株即为1号生理小种的毒性纯系。

Article 12 A person having a quarantinable infectious disease shall be placed in isolation by the frontier health and quarantine office for a period determined by the results of the medical examination, while a person suspected of having a quarantinable infectious disease shall be kept for inspection for a period determined by the incubation period of such disease.

第十二条国境卫生检疫机关对检疫传染病染疫人必须立即将其隔离，隔离期限根据医学检查结果确定；对检疫传染病染疫嫌疑人应当将其留验，留验期限根据该传染病的潜伏期确定。

Article 12 A person having a quarantinable infectious disease shall be placed in isolation by the frontier health and quarantine office for a period determined by the results of the medical examination, while a person suspected of having a quarantinable infectious disease shall be kept for inspection for a period determined by the incubation period of such disease.

第十二条国境卫生检疫机关对检疫传染病染疫人必须立即将其隔离，隔离期限根据医学检查结果确定；对检疫传染病染疫嫌疑人应当将其留验，留验期限根据该传染病的潜伏期确定。英文翻译，胜任简体和繁体五年经验，擅长财经、法律类

A person having a quarantinable infectious disease shall be placed in isolation by the frontier health and quarantine office for a period determined by the results of the medical examination, while a person suspected of having a quarantinable infectious disease shall be kept for inspection for a period determined by the incubation period of such disease.

第十二条国境卫生检疫机关对检疫传染病染疫人必须立即将其隔离，隔离期限根据医学检查结果确定；对检疫传染病染疫嫌疑人应当将其留验，留验期限根据该传染病的潜伏期确定。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。