疫

The rationality is discussed that fatigue life and fatigue crack growth rate determined in impact fatigue testing are used as a measure of fatigue cracking resistance of hot drop-forging die steel.

本文探讨了用冲击疫劳试验测定的疲劳寿命和疲劳裂纹扩展速率衡量热锤锻模材料疲劳抗力的合理性。

Some differences of pathogenicity of different cotton isolotes were observed on cotton seedlings, but not on cotton bolls.

来自棉花上的不同苎麻疫霉菌株对棉苗的致病力存在一定的差异，但对棉铃的致病力差异不明显。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

How often have you heard of persons being killed by drownings, by fatal falls from high places, of persons dying at meals, at play, in fires, by the sword, in pestilence, or at the hands of robbers!

你有多少次，听得说过：某人被刀砍死，某人落水淹死，某人从高处跌下，头破而死，某人吃饭的时候死了，某人游玩的时候死了。这一个死於火，那一个死於枪，有人染疫而死，也有人被盗贼害死。

The quarantine signal of a ship is in the day time to fly a signal flag of international code,"Q"flag signifies that the ship is not infected, requesting the issuance of an entry pratique

昼间在明显处所悬挂国际通语信号旗：&Q&字旗表示：本船没有染疫，请发给入境检疫证。

That forest-dell, where Lowood lay, was the cradle of fog and fog-bred pestilence; which, quickening with the quickening spring, crept into the Orphan Asylum, breathed typhus through its crowded schoolroom and dormitory, and, ere May arrived, transformed the seminary into an hospital.

罗沃德所在的林间山谷，是大雾的摇篮，是雾气诱发的病疫的滋生地。时疫随着春天急速的步伐，加速潜入孤儿院，把斑疹伤寒传进了它拥挤的教室和寝室，五月未到，就己把整所学校变成了医院。

Jacob Halsey, in charge of the Fort Union depot, thought the best thing to do was to vaccinate everybody and he is said to have been surprised when a number of his subjects began vomiting, bleeding, and dying.

而居住在密西西比河上游的联邦堡地区的印第安人，不顾及知道病疫危险的白人们的警告，拒绝从船边离开，拒绝迁散，认为这是再次对他们的欺骗。那时联邦堡补

Gray mold of epidemic disease of Chinese cabbage soft rot, green pepper, tomato is alternant indulge in wilful persecution, the dish in the ground rots is.

白菜软腐病、青椒疫、西红柿灰霉病交替肆虐，地里的菜一烂就是一片。

MethodsFive indexes, ShannonWeaner diversity value, Pielou uniformity value, dominance value, Levins niche width value and Petraitis niche overlap value were calculated to describe the community structure and niche characteristics of the HFRS host rats in the experimental districts.

方法以ShannonWeaner的群落多样性指数、Pielou群落均匀度指数、优势度指数、Levins生态位宽度指数、Petraitis生态位重叠指数定量描述疫区内HFRS宿主鼠类的种群结构及生态位特征。

This study served as a basic research for resistance breeding,a study of factors which infleunced the growth of myceliuni of Phytophthora sojae and its zoospores production was carried out basedon R25 of? sojae.

本文围绕抗病育种这一中心，以大豆疫霉根腐病病原菌25号生理小种，对影响其菌丝体生长和游动孢子产生的因子进行了研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。