疫

The specific PCR products of approximately 112bp in length was amplified from the other 10 strains of E.faecalis from lamb naturally infected with E.faecalis,lamb and mouse experimentally infected with E.faecalis by the PCR assay,but no any bands were amplified from Streptococcus equi subsp.equi,Streptococcus G,Streptococcus A,Staphylococcus aureus,Listeria monocytogenes from sheep,Mycoplasma ovipneumoniae and Brucella ovis.

应用该方法对分离的致羔羊脑炎粪肠球菌、由肠球菌导致发病或死亡羔羊脏器以及人工感染死亡小鼠的主要脏器进行扩增，均能得到特异性的DNA条带；对其他7种病原菌，马腺疫链球菌C群、G群链球菌、A群链球菌、金黄色葡萄球菌、羊源李氏杆菌、绵羊布氏杆菌、绵羊肺炎支原体不能扩增出特异的DNA条带。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Objective: To study the disposition of epidemic focus and species of Paragonimiasis and infection condition of people, and the independence of P.

目的：研究福建省三元区并殖吸虫病疫源地的虫种分布和人群感染情况，并通过形态观察和分子生物学鉴定研究泡囊狸殖吸虫的独立性。

There are many endemic areas of Paragonimus in Jiangxi Province, however, the distribution of Paragonimus has been gradually limiting to mountainous areas.

江西境内存在众多的并殖吸虫自然疫源地或流行区，但其分布发生了变化。

Constitution and distribution of freshwater crabs have close relationship with altitude, terrain features, and movements of water in the area. Wild rodent animal may contribute to the maintenance and expansion of the endemic areas of Paragonimus .

淡水蟹类种类的组成与分布与其不同海拔高度和地势地貌及其水系的走向等密切相关；野生啮齿类动物对于并殖吸虫疫源地的维持甚至扩大起着一定的作用。

DsRNA viruses of C. parasitica from east of China were divided into 4 types on the basis of electrophoresis patterns.

采用生物学测定和凝胶电泳对栗疫病菌的dsRNA进行研究。

The spatial structure of genetic diversity among 17 populations of Cryphonectria parasitica in China was investigated using RAPD markers with the spatial autocorrelation analysis.

应用空间自相关分析方法对中国栗疫病菌 17个居群 RAPD遗传变异的空间结构进行研究，以探讨栗疫病菌居群遗传变异的分布特征及其形成机制。

The results showed that 9 strains presented cytotoxic activities to at least one of the tested tumor cells, five strains exhibited strong antimicrobial activity against Staphylococcus aureus, one strain had inhibitory effect on Phytophthora parasitica.

结果显示，有9株内生真菌至少对一种指示瘤株具有细胞毒活性， 5株内生真菌对金黄色葡萄球菌有较强的抑菌活性， 1株内生真菌对辣椒疫霉有抑制作用。

Using 11 primers filtrated from total 53 primers, ISSR amplification was conducted in 220 individuals from eleven Cryphonectria parasitica subpopulations which were from four countries, and 174 polymorphic loci were detected from total 177 loci. The percentage of polymorphic loci of the total populations was 98.31%, while that at a subpopulation level averaged 20.43 %. Shannon's Information index and Nei's gene diversity of the fungus at the total populations level was 0.1769 and 0.7386, respectively.

自53个ISSR引物中筛选出11个引物，对以上四个群体的栗疫病菌11个子群体的220个菌株进行扩增，共检测到177个位点，其中多态位点为174个，多态位点百分率为98.31%。11个栗疫病菌子群体的P平均值为20.43%；群体水平的Shannon信息指数和Nei指数分别为0.1769和0.7386；各个子群体I的平均值为0.0943，h的平均值为0.0614。

Secondly, the rate of infection of the Pasteurella multocida reached to 16%(12/75), Riemerella anatipestifer bacilliis also high, the ratio was 12%(9 / 75). More over, hemolytic bus bacteria, Salmonella and Staphylococcus aureus were also showed in the poultry tested.

结果表明：大肠杆菌仍是该部分地区危害家禽的主要致病菌，它的个体感染率高达41.3%（31/75）；其次是多杀性巴氏杆菌，个体感染率为16%(12/75)；鸭疫里默氏杆菌的个体感染率也较高，为12%（9/75）；此外还分离到溶血性巴氏杆菌、沙门氏菌和葡萄球菌。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。