疫

The inheritance and variation of the biological characters including colony morphology, growth rate, homothallism, pathogenicity, metalaxyl-resistance and antheridium attachment mode of Phytophthora boehmeriae Sawada, the important homothallic Phytophthora species causing cotton and ramie blights in China, were reporteed in this paper.

本文首次对引致我国棉花、苧麻疫病的重要同宗配合疫霉种——苧麻疫霉的菌落形态、生长速率、同宗配合特性、致病力、对甲霜灵抗性以及雄器着生位置等生物学性状的遗传与变异进行了较系统的研究。

Results Five DFRs were found. One of the DFR of 383 bp was specific to biovar antiqua. The 383 bp fragment was only found in strains of Y.pestis isolated from Marmota baibacina Spermophilus undulates Plague Focus of the Tianshan Mountains, but not found in strains from other Plague Foci. It was also found in two (belonging to biotypeⅠ and Ⅱ) of five strains of Y.pseudotuberculosis .

结果 发现了5个在不同生物型鼠疫菌间存在差异的DNA片段，其中一个383bp的片段在来自天山山地灰旱獭、长尾黄鼠鼠疫自然疫源地的30株鼠疫菌全部阳性，而来自其它鼠疫自然疫源地的菌株全部阴性，5株假结核耶尔森菌中有2株阳性。

FMD is a virulent infectious disease caused by FMDV that infects artiodactyl such as cattle, sheep, pig, et al. Outbreak and prevalence of the disease endanger the good development of graziery seriously and cause great financial loss, so all the world highlight study and contol of the disease.

口蹄疫(Foot-and-Mouth Disease，FMD)是由口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)引起的牛、羊、猪等偶蹄动物感染的一种烈性传染病，该病的发生和流行严重危害畜牧业的健康持续发展，造成巨大的经济损失，因此世界各国相当重视对该病的研究和防治。

FMD is a virulent infectious disease caused by FMDV that infects artiodactyl such as cattle, sheep,pig, et al. Outbreak and prevalence of the disease endanger the good development of graziery seriouslyand cause great financial loss, so all the world highlight study and contol of the disease. Material of the study was FMDV from cell culture.

口蹄疫(Foot-and-Mouth Disease，FMD)是由口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)引起的牛、羊、猪等偶蹄动物感染的一种烈性传染病，该病的发生和流行严重危害畜牧业的健康持续发展，造成巨大的经济损失，因此世界各国相当重视对该病的研究和防制。

On the basis of the analysis of the change of the natural epidemic source and disease tendency in the part of the region of Hubei province, the influences of diversion project from south to north to the diseases, such as local fluorosis, blood fluke and so on, are pointed out and some suggestions are proposed.

本文在分析湖北省局部地区自然疫源变化及疾病趋势的基础上，指出了南水北调中线工程可能对地氟病、血吸虫、疟疫、乙脑及其它水源性疾病所带来的影响，并提出了建设性的建议和对策。

The pathogen causing lily blights was isolated from diseased lily with typical symptoms on basal stem. The pathogen was identified as Phytophthora nicotianae van Brede de Haan according to the characteristics of mycelium, chlamydospore, zoosporangium, oospore and the test of pathogenicity of the pathogen.

从具典型症状的新鲜百合疫病植株茎基部病组织中分离到百合疫霉菌，根据其病原菌菌丝的形态、菌落特征，厚垣孢子、游动孢子囊和卵孢子的形态和大小，以及病原菌致病性测定，该病原菌鉴定为烟草疫霉 Phytophthora nicotianae van Brede de Haan。

There are also foci mainly with campestral hosts of Apodemus agrarius and Apodemus draca and Eothenomys miletus in the monitoring areas.

监测区内存在以褐家鼠和黄胸鼠为主要宿主的家鼠型疫源地，也存在着以高山姬鼠、中华姬鼠和大绒鼠为主的野鼠型疫源地。

Boehmeriae were studied, and the results indicated that the zoospores of P. boehmeriae could be inactivated obviously by irradiating with 30W ultraviolet light 50cm away from the culture dishes tested and that 95% germination of zoospores could be restrained by 30W ultraviolet light treatment for 40sec.The mycelium growth from mycelium discs of P. boehmeriae were inhibited mostly by 30W ultraviolet light treatment for 20min. Afterwards, the inhibitive rates were decreased with the rise of the treatment time, which showed that the mycelium discs of P. boehmeriae had produced flexibility to ultraviolet rays after being irradiated by certain dosage of ultraviolet light.

研究了紫外光对苎麻疫霉生物学特性的影响，结果表明，30W的紫外灯管距培养皿50cm照射处理对苎麻疫霉的游动孢子具有明显杀灭作用，紫外光照射40sec可以抑制95%的游动孢子萌发；紫外光照射20min对菌丝块的菌丝生长的抑制率达最大，以后随着处理时间增加，抑制率却有降低的趋势，说明苎麻疫霉的菌丝块，经一定剂量紫外光照射后对紫外线产生了适应性。

The results showed that Phytophthora sojae on the cellophane carrot agar could not produce oospores within 10 days, no medium were attached when Phytophthora sojae was taken away from cellophane, high-quality RNA, which could meet the requirement of differential display reverse transcriptional PCR was obtained, indicating that it was a suitable cultivation method for Phytophthora sojae to produce pure vegetative mycelia.

结果表明，玻璃纸胡萝卜琼脂培养的大豆疫霉菌，10 d内不会产生卵孢子，当大豆疫霉菌从玻璃纸上取下时，不会带有培养基，能够提取出高质量的RNA，满足差异显示反转录PCR对RNA质量的要求，是一种适合大豆疫霉菌纯营养菌丝的培养方法。

Culture medium, water volume, root exudates of different crops, temperature, illumination affecting germination conditions of oospores of Phytophthora sojae studied in this reasch and compared the difference of germination of oospores of different race of Phytophthora sojae, filtrated the higher germination rate of strain and suitbable conditions combination of germination of oospores; The pure-line(Race 1) in virulence was obtained through isolation and culture and virulence identify of the second generation. The pure-line was analyzed by molecule method of ISSR. The results were summarized as follows:There was quite difference of germination rate of oospores on different culture medium.

本文测定了不同培养基、水量、农作物根渗出液、温度、光照对大豆疫霉菌卵孢子萌发影响，比较了大豆疫霉菌株间卵孢子萌发的差异，筛选出萌发率较高的菌株和适于卵孢子萌发的条什组合；通过继代单卵孢株分离培养和毒性鉴定，建立了大豆疫霉菌单卵孢株毒性纯系，并分析了单卵孢株毒性纯系后代群体ISSR分子指纹，结果如下： 1。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。