疟原虫

Objective To identify the genotype of merozoite surface protein 1 (MSP1) of Plasmodium falciparum isolates from Hainan Province.

目的 了解海南省恶性疟原虫裂殖子表面蛋白 1(PfMSP1)等位基因型的分型特征。

Objective: To clone whole merozoite surface protein-2 (MSP2) gene of Plasmodiutn falciparum and express it in E.

目的：克隆恶性疟原虫裂殖子表面蛋白2全基因，并在大肠杆菌中进行表达。

Merozoite Surface Protein 1 of Plasmodium falciparum...

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

Merozoite Surface Protein 1 of Plasmodium falciparum(PfMSP1) has been considered a leading candidate for malaria vaccine.

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

Merozoite Surface Protein 1 of Plasmodium falciparum (PfMSP1) has been considered a leadi.

恶性疟原虫主要裂殖子表面蛋白1（PfMSP1）是当今领先的疟疾疫苗候选抗原，其主要实验依据是来自夜猴攻击试验。

After immunization, kinetics of antibody response, isotype of IgG antibody, and antibody responses to the individual components of the chimeric antigen were detected by ELISA. Interactions of the antibodies with native antigens located on surface of merozoite of Plasmodium falciparum were analyzed by indirect immunofluorescent assay.

以ELISA检测免疫血清中特异性抗体的动态变化、IgG抗体亚型及对融合抗原各组分的抗体水平，以间接荧光抗体实验分析免疫血清对恶性疟原虫天然抗原的识别情况。

P.vivax ; merozoite surface protein 1（MSP1）; genotype ; morphology

间日疟原虫；裂殖子表面蛋白1；基因型；形态学

Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064（pQEM1）strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 （MSP1）,the stability of its plasmid, and its re-expression.

目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力，所含质粒的稳定性和再表达能力。

AIM: To analyse the humoral immune response in mice to nucleic acid vaccines ( VR1012/HG MSP1 17 for intracellular expression or VR1012/TPA/HG MSP1 17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL 1 and TT.

目的： 检测以恶性疟原虫裂殖子表面蛋白1 的17 区片段基因为基础的复合核酸疫苗VR1012/TPA/HG-MSP1-17和VR1012/HG-MSP1-17诱导小鼠的体液免疫反应。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。