疟原虫

However, there are still several safety concerns about the use of a DNA vaccine, which include the possibility of integration into the host genome, adverse immunopathology, and anti-DNA autoantibody induction.Attempting to solve some of these problems, eukaryotic expression plasmids pTL-8/ AMA-1 and pTL-8/ AMA-1 which express trans-activator or reverse trans-activator, respectively, and AMA-1 gene of Plasmodium falciparum were constructed by using tetracycline regulable system.

首先构建了恶性疟原虫顶第四军医大学硕士学位论文端膜抗原1(AMA一1)基因和反式活化因子基因的真核表达质粒pTL一8/灿认一l和PTL一8/灿认一1，并大量制备这两种质粒及表达转录抑制子的质粒pUHS6一1。

Using the method of edge and gray scale detection, the Plasmodium vivax schizont can be detected and identified.

初步探索采用边缘和图像灰度联合检测的方法能够识别间日疟原虫薄血膜的裂殖体。

The transcription of PfRON4 peaks at mature schizont stage, suggesting that the PfRON4 gene may involve in erythrocyte-invasion of P.

定量分析结果显示，在恶性疟原虫红内期发育过程中， PfRON4基因的转录水平在裂殖子入侵红细胞后36～40 h达到高峰。

The mature or nearly mature schizont often was often not observed the cytoplasm.

斑鸠疟原虫无性体也较大，成熟裂殖体一般有20个以上的裂殖子，而且一般观察不到细胞质。

Methods We processed the image of thin smear film of the Plasmodium vivax schizont through several steps including image preprocessing, image segmentation and feature extraction. The recognition rate and accuracy were observed.

基于图像预处理、图像分割、特征提取、判决分类等步骤，分别对间日疟原虫薄血膜的裂殖体图像进行处理，观察其识别效率和准确度。

Results The sporogony of Plasmodium cynomolgi can be inhibited within the doses of 1.8～ 15.0mg/kg.

结果在1.8～15mg/kg剂量范围内均能阻断食蟹猴疟原虫孢子增殖。

An infected female Anopheles mosquito's saliva contains great numbers of the form of the parasite known as the sporozoite.

在感染了的雌疟蚊的唾液里，含有大量叫作子孢子的疟原虫。

Methods Entire coding sequence of CSP was reverse transcribed and amplified by RT-PCR with sporozoite total RNA as template,then cloned into pFLAG-CMV8 for construction of the recombinant plasmid pFLAG-CMV8-CSP.

以约氏疟原虫BY265株子孢子总RNA为模板，用RT-PCR扩增CSP基因的编码区序列并克隆至pFLAG-CMV8载体，构建重组质粒pFLAG-CMV8-CSP。

Objective To investigate the effect of daphnetin on superoxide dismutase activity and DNA synthesis in P. falciparum in vitro.

目的研究瑞香素对体外培养恶性疟原虫超氧化物歧化酶活性及DNA合成的影响。

Methods: The SSUrRNA gene of tertian malaria parasite was used as indication molecule. End-labelling method was used for biotin labelling.Immuno-PCR was developed based on the principle of ELISA and PCR.

以间日疟原虫SSUrRNA基因为指示分子，采用生物素进行末端标记，利用酶联免疫吸附试验和PCR原理，建立免疫-PCR法，并对60份临床标本进行检测。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。