疟原虫

We found some Pfdyn cDNA fragments in Plasmodium falciparum 3D7 gametocyte cDNA library and some Pfdyn peptide fragments in merozoite and gametocyte.

通过对疟原虫基因组数据分析，又找到另一个属于动力素家族的恶性疟原虫基因，Pfdyn2，与Pfdyn蛋白有38％的同源性。

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1／HN株的己糖激酶编码基因，构建其原核和真核表达重组质粒，测定其序列，比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1(MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition.

摘 要：目的检测以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础的复合核酸疫苗(分泌性的VR1012/TPA/HG-MSP1-17和非分泌性的VR1012/HG-MSP1-17)诱导小鼠的体液免疫反应和免疫血清对疟原虫生长的抑制能力。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Blood smears with discrepant results between the two methods were retested by an experienced microscopist,and also repeated by UT-PCR for 2-3 times. The sensitivity and specificity of the two methods were evaluated following the Tjitra′s method.

结果 400例发热患者血样中，镜检法初检检出疟原虫阳性234例，其中恶性疟125例，间日疟109例；UT-PCR检出疟原虫阳性235例，其中恶性疟124例，间日疟109例；恶性疟和间日疟混合感染2例。

The effect of tat p53 on the growth of parasites was determined by counting the parasitemia changes between pre and post incubation of the fusion protein with the parasite culture for 12 h. results: tat p53 fusion protein could be transduced into p.f parasites, locating at trophozoit and late schizont stage, whereas p53 protein could not.

结果：tat p53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

The effect of TATp53 on the growth of parasites was determined by counting the parasitemia changes between pre and postincubation of the fusion protein with the parasite culture for 12 h. RESULTS: TATp53 fusion protein could be transduced into P.f parasites, locating at trophozoit and lateschizont stage, whereas p53 protein could not.

结果：TATp53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

The sporozoite rates of An.minimus,An.anthropophagus,An.sinensis and An.candidiensis were 0.08%-8.11%,0.25%-1.85%,0.005%-0.32% and 0.06%-3.22%,respectively.

历史上广西是我国南方四大高疟区之一，流行的疟原虫种类有恶性疟原虫、间日疟原虫和三日疟原虫。

Results: DNA content of the plasmodia not treated with any drugs was not changed in 24 hours,while benflumetol could decrease the DNA content: the DNA content began to decrease 2 h after the drug administration and reached the minimum by 16 h, but somewhat increased at 24 h after administration.

结果：对照组疟原虫DNA含量在各时间点没有显著变化。本芴醇单次给药后，随时间推移疟原虫DNA含量逐渐减少，药后2 h两给药组疟原虫红内期 DNA含量开始降低，到16 h降到最低，但药后24 h DNA含量又有所回升。

Malaria caused bu Plasmodium vivax, Plasmodium ovale and Plasmodium malariae causes milder disease in humans that is not generally fatal.

疟疾造成的布疟原虫，卵形疟原虫和三日疟原虫引起温和人类的疾病，通常不会致命。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失