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We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时,我们以人类CNE1细胞系为材料,比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法,发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍,并且这些整合膜蛋白的疏水值大部分都为正值。

We confirmed that the adjacent layer interpenetration is severe and the re-organization of the components occurs in the deposition process.

首次利用动态接触角法跟踪自组装膜的形成过程,阐明了聚电解质组分在膜上的穿插重排行为,疏水相互作用对膜形成的贡献,组装条件尤其是水洗和干化对自组装的影响。

In comparison with normal IgG, the hydrophobic groupsof SLE IgG molecules resided at the positive electricity regions and were exposed more to the surface of lgG molecules.

同正常人相比,SLE患者血清IgG园二色谱中213nm处负峰的椭园值比正常人的高出近一倍,IgG分子中的疏水基团处于正电区,并且趋向于分布在蛋白质分子的表面。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的,尽管硫氧还蛋白有很强的助溶作用,该蛋白依然不可溶形式存在;NS5B—dc21蛋白通过C末端疏水序列的删除,获得了可溶表达;NS5AB蛋白以不可溶形式表达,需要复性。

The influencing factors that glutathione molecular umbrella penetrates lipid bilayers with the shielded conformation were analyzed with VolSurf parameters. The results show that the major influencing factors are smaller amphiphilic moment and larger molecular rugosity of the shielded conformation, which are independent of definite hydrophobic regions of the conformation.

利用VolSurf参数分析了分子伞以屏蔽构象穿透磷脂双分子层的影响因素,结果表明屏蔽构象较小的两亲矩及较大的分子褶皱程度是其能够穿透细胞膜的主要影响因素,与构象的绝对疏水区域无关。

This pressure/temperature relationship causes the duplex diaphragm to expand uniformly, closing the orifice opening in a replaceable stainless steel seat with a self-centering corrosion-resistant and hardened stainless steel diaphragm valve.

这种压力/温度关系导致双隔膜能够以相同的速度膨胀,关闭可更换不锈钢阀座内的节流孔的开口。这种疏水阀配置能够自动置中的耐腐蚀硬化不锈钢隔膜阀。

Our plant specializes in producing drain valve and small-bore forging steel valve with long-standing history,advanced technology,comprehensive inspection technology and standardized quality control.

本厂是专业生产疏水阀和小口径锻钢阀门的厂家,具有悠久的历史,先进的工艺、完善的检测技术、规范化的质量管理。

No squalene synthase polypeptide of expected molecular mass was observed in E. coli containing the putative full-length squalene synthase cDNA, however, overexpression in E. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus.

但在含有全长的鲨烯合酶cDNA的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达,而C末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。

At high pH value, histidine is transformed into a bidentate ligand after the deprotonization of its R-NH2, and thus the aggregation process is suppressed greatly.

这是由于多肽二级结构转化是一个协同性过程,只有pH值和温度的协同作用,才能诱导具有疏水特性的β-折叠片结构的形成,从而导致金纳米粒子的聚集和颜色变化。

UV-Vis and continuous variation plot method were used to study the complexation of γ-cyclodextrin with bromocresol green, and the stoichiometric ratio of the complexation was to be 1∶2. Thermodynamic analysis results show an inverse relationship between temperature and complexation constant, and the change of enthalpy, entropy and free energy of the complexation were -39.988 kJ/mol, 86.400 J/ and -14.245 kJ/mol, respectively, which indicating that hydrophobic effect was the main force to form the complexes.γ-Cyclodextrin and bromocresol green complexes were examined by nuclear magnetic resonance, infrared spectrum and molecular modeling analysis, and was may be the group included in γ-cyclodextrin.

采用紫外-可见分光光度法和等摩尔连续变化法研究了γ-环糊精与溴甲酚绿的包合作用,确定了包合物形成的化学计量比为1∶2;采用热力学方法分析了温度与包合常数之间的关系,计算了包合过程的焓变、熵变及自由能变化分别为-39.988 kJ/mol, 86.400 J/和-14.245 kJ/mol,这表明疏水作用力为主要驱动力;采用核磁共振、分子模拟和红外光谱法对包合物进行了研究,确定了包合物的形成,分析认为这可能是基团进入γ-环糊精腔内导致增色效应。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。