疏

Over the past five years, President Chirac has kept him at arm's length, passing him over for the job of prime minister, grooming alternative Gaullist heirs and inventing rules to try to keep the centre-right party out of his hands.

13%的选票。过去五年中，希拉克总统对其疏而远之，将总理之位授予他人，选荐培养另外的戴高乐派接班人，还创造出些条款试图阻止中右政党落入其手。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

His paintings about flowers and birds were distinctive and full of sentiment in the painting world in Yangzhou during the 18thc,whose artistic style is fresh,clear,straightforward,sparse and thin,gentle and simple.

华喦花鸟画在18世纪扬州画坛，以其清新隽秀、率意疏宕、文质相兼的艺术风格，表现出与众不同且极具情趣的个性化特征。

Objective To study the protective effect and mechanism of Shuxuetong on gerbil brain tissue in the area of ischemia reperfusion.

目的 探讨疏血通对沙土鼠缺血再灌注脑组织的保护作用及其机制。

The results show that the interaction between ligand and γ-globulin includes mainly electrostatic and hydrophobic interaction.

研究结果表明，BGG与氨基酸之间的亲和相互作用力主要是静电力和疏水相互作用力。

In addition, the glycerol or sucrose effect led to the alteration of the

同时，甘油和蔗糖的作用也会导致PSI的疏水性增加。

The hydrophobic patch including Pro40 residue plays an important role on the golbal structure of Cyt 〓.

包括Pro40在内的疏水斑区在稳定细胞色素〓的整体结构方面起着极为重要的作用。

Corresponding measures are proposed to protect well field and water environment by setting well field gradational protection system, and expl...

提出了建立水源地分级保护体系、以拦蓄排泄带方式开采地下水等保护水环境的应对措施；还介绍了防治或减轻含水层结构破坏的"开采技术措施"；在一定措施控制下的"含水层再造技术"；在烧变岩带采用"疏排型水源地开采技术"；"排水-供水-生态环保三位一体结合技术"以及针对矿区周围沙地较多特点的"矿井水沙地净化处理技术"。

We compared the number of integral membrane proteins identified from the PM of human CNE1 cells by PAOT with that of an optimized trypsin/chymotrypsin method. More than double the total number of identified IMPs was obtained with PAOT, mainly contributed by the IMPs with positive grand average of hydropathicity scores.Part 2: We describe a procedure for digesting the hydrophilic domains in the absence of detergents and denaturants.

同时，我们以人类CNE1细胞系为材料，比较了PAOT与另外一种优化好的胰蛋白酶/胰凝乳蛋白酶酶解方法，发现PAOT鉴定到的整合膜蛋白是胰蛋白酶/胰凝乳蛋白酶酶解的2倍，并且这些整合膜蛋白的疏水值大部分都为正值。

A strange doom is thy father's sons's, and past Recalling, as it lies beyond redress; Reversed for him our grandsire's fate of yore,- He had no rest at sea, nor I on shore.

如果我有了后者，前者就不值一提，你会成为我的幸福之避难的港湾；但是，还有许多别的关系系住你，我不原意你因为我而和一切疏淡。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。