番木瓜蛋白酶

It is very difficult to isolate chymopapain from the latex of Carica papaya because chymopapain is very similar to the other three cysteine proteinases in the latex at the structure and properties.

由于番木瓜乳汁中含有的四种巯基蛋白酶在结构、性质等方面十分相似，因而分离木瓜凝乳蛋白酶比较困难。

Methods Using means of collection, 20% and 40% saturated ammonium sulfate fractionation, SP-sephadex C 50 column chromatography and hydroxyapatite chromatography to purify papain from Carica papaya.

采用初步采集处理，20%，40%硫酸铵分级沉淀、SP-sephadex、C50柱色谱、羟基磷灰石柱色谱，从番木瓜乳汁中分离纯化木瓜蛋白酶。

Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录，得到木瓜凝乳蛋白酶的cDNA，PCR产物克隆到pET22-b载体中，重组质粒转化至大肠杆菌BL21，进行诱导表达及表达产物的检测。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。