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A capillary electrophoresis fingerprints method was established to evaluate the quality of Belamcanda chinensis DC. The background electrolyte was a 80 boric acid containing 15 borate solution (adjusted pH 9.7 with KOH solution).

采用毛细管区带电泳法,以80mmol/L硼酸 15mmol/L硼砂缓冲液(用氢氧化钾溶液调pH9 7)为背景电解质,运行电压12 1kV,检测波长228nm,重力进样10s(高度7cm),建立了射干药材的毛细管电泳指纹图谱。

All of them were labeled with [32P] orthophosphate (2.03×10^5 Bq/mL) for 2 h and then stimulated with CCK8 l0^(-7 mol/L and DMEM respectively for different time. Reactions were terminated by freezing neurons in liquid nitrogen. The 32P-labeled lysates isoelectrically focused on IPG Drystrip pH 4~7 linear gels and subsequently separated by second-dimensional SDS-PAGE.

培养乳小鼠神经元,随机分为CCK8刺激组和对照组,32P-NaH2PO4标记后分别给予CCK810^(-7 mol/L和等量无磷培养基,作用不同时间后液氮终止反应,经双向电泳、放射自显影,获得磷酸化蛋白质的电泳图谱。

Methods By consecutive electrophoresis and nonconsecutive electrophoresis experiments, the best analysis conditions of chitooligosaccharide were determined.

通过连续电泳和不连续电泳对比实验确定壳寡糖的最佳分析条件。

Based on noncontinuous spray for each sprayer, the multiplex low flow system was achieved by applying ESI potential sequentially to the four low flow sprayers, resulting in only one sprayer being sprayed at any given time.

为了使低流速鞘流界面可被应用在多通道毛细管电泳质谱,我们利用非连续电喷洒的概念,使此四根毛细管电泳产生序列式喷洒,再与质谱仪同步化。

The c oaventioaal method was improved by using agarose as supporting medium instead pured agar and adopting pre-electrophoresis in order to make it more sensitive.

笔者在常规方法的基础上,做了些改进:用琼脂糖代替纯化琼脂;并先将诊断血清加在阳极端孔内,预电泳15—20分钟,然后将待检标本加入阴极端孔内继续电泳50分钟。

Seven isozymes were analyzed by means of horizontal sliceable starch gel electrophoresis.

采用水平切片淀粉凝胶电泳检测了七种同工酶的酶谱图式,从中筛选出酯酶同工酶进一步作聚丙烯酰胺凝胶电泳分析。

Methods: SDS-polyacrylamide gel eletrophoresis was employed to analyze the seed protein of different cultivars and wild plant of Fructus Trichosanthis from Shandong and Trichosanthes hubehensis, and the finger-prints were established.

利用垂直平板聚丙烯酰胺凝胶电泳技术,对山东产瓜蒌的不同农家品种、野生种及湖北栝楼进行种子蛋白电泳分析,并绘制指纹图谱。

In the present study, efficient identification methods for the main varieties of cowpea (Vigna unguiculata W.) and cauliflower (Brassica oleracea L.) in south of China, especially in zhejiang province, were investigated by using seven cowpea varieties (variety 1, Zhijiangtechang80;variety2, Zhijiangtechang90;variety3, Qiujiang512;variety4, Zhijiang228-2;variety5, Huajiang24;variety6, Yangjiang40;and variety7, Xingaochan8) and five cauliflower varieties (variety A, Longfenteda50tian;variety B, Yidaijinguang80tian;variety C, RuixuetedalOOtian;variety D, Qiuwang80tian;and variety E, Tezao50tian). The application of seed shape, seedling shape, rapid chemical identification and electrophoresis was performed, the main results were as follows.

为开展南方特别是浙江省主要蔬菜作物种子高效快速鉴定技术研究,本试验以七个豇豆品种品种1(之豇特长80,品种2(之豇特长90),品种3(秋豇512),品种4(之豇228—2),品种5(华豇24号),品种6(扬豇40号),品种7和五个花椰菜品种品种A(龙峰特大50天,品种B(一代金光80天),品种C(瑞雪特大100天),品种D(秋王80天),品种E(特早50天)为材料,从种子形态、幼苗形态、快速化学和电泳鉴定等方面对豇豆和花椰菜品种鉴定进行了研究,特别是对乳酸—聚丙烯酰胺凝胶电泳在这两种蔬菜品种鉴定上的可行性进行了研究。

Methods EST isozymes of three blackfly species were studied by discontinuous PAGE techniques. Results Ten isozyme bands could be identified according to the electrophoresis zymograms.

应用垂直板不连续聚丙烯酰胺凝胶电泳技术,对素木蚋、双齿蚋和五条蚋进行EST同工酶电泳,测量并计算各酶带的迁移率。

Experiments was performed on the cathode electrophoresis paint performance and pretreatment process.

研究了新型低温固化低密度阴极电泳漆(KNT831)的性能。4。对阴极电泳涂料性能及前处理工艺进行了试验。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?