电泳的

The method was also applied to study the scavenging activity of angelica polysaccharide.

在第二节中探讨毛细管电泳-安培检测中的等速电泳富集现象。

Methods The total protein of the cashew nut was isolated by SDS-PAGE. The allergens of cashew nut were identified by Western-blotting. To purify the allergens, ion exchange chromatography was employed. Results There were ten protein bands visible on the SDS-PAGE gel.

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳电泳分离腰果的蛋白质组份，采用免疫印迹(Western-blotting)方法鉴定过敏原，通过离子交换层析对腰果主要过敏原进行初步纯化。

The paper is about the improvement of the method of isolating serum protein by acetate cellulose membrane,in which the experimental result as well as the zone is clearer.

本文重点对醋酸纤维薄膜电泳分离血清蛋白实验的电泳条件及透明部分作了一些改进，使实验结果较以前清晰，区带明显。

The changes of nutrition in milk treated with different technologies are also determined. Results showed that, 1 higher pressure, longer holding time and more alternative times favour a larger destruction of microorganisms; 2 the combined effect of hurdle technology and pressure on the destruction of microorganisms in fresh raw milk is more effective than only using pressure treatment, among which the effect of ε-polylysine on inactivation of microorganism in fresh raw milk is more effective than that of Nisin with the same concentration of 100mg/L; 3 the whole proteins in milk treated by the different types of sterilization have been detected by SDS-PAGE electrophoresis, and comparing with the whole proteins' electrophoretogram of fresh raw milk, we found that the first spectrum band near the negative pole was disappeared and the colours of the second spectrum band was tint in UHT, pasteurism and 500MPa treated milks, which was suggested that some proteins' subunits were dissociated, but the 400MPa treated milk showed the similar eletrophoretogram with the fresh raw milk; 4 the loss of threonine, VA and VC in high pressure treated milk was more less than those in pasteurism treated milk, in particular threonine and VA were almost retained, and the contents of VB1 and VB2 in the former are also more than that in the latter; 5 high pressure treated milk had longer shelf life than pasteurism treated milk, extending the sale semidiameter of milk.

压力越高，保压时间越长，交变次数越多，杀菌效果越好；2）采用栅栏技术对牛乳中的微生物具有更有效的杀菌作用，其中相同浓度的（终浓度100mg/L）ε-聚赖氨酸较Nisin具有更强的杀菌效果；3）利用SDS-PAGE电泳研究了不同杀菌方式对牛乳总蛋白的影响，发现经UHT、巴氏和500MPa交变处理的牛乳与生鲜牛乳相比其总蛋白电泳图谱靠近负极方向第一条谱带消失，第二条谱带颜色变浅，由此推测经上述处理后牛乳中有一些蛋白质的亚基发生了解离，而经400MPa交变处理的牛乳与生鲜牛乳的总蛋白电泳图谱相似；4）超高压牛乳营养成分与生鲜牛乳相比，苏氨酸、VA、VC在超高压处理中受到的破坏明显比巴氏杀菌小得多，特别是苏氨酸和VA几乎完全被保留下来，而VC含量也要比巴氏奶高出25%以上，对于VB1、VB2，检测数据也表明，超高压灭菌对其破坏要比巴氏灭菌小，其含量分别比巴氏杀菌牛乳高出9.5%和2.6%；5）与巴氏杀菌乳相比，超高压杀菌有助于延长牛乳的保质期，扩大牛乳的销售半径。

The research displayed that yeasts Genus level can be discerned by agarose gel electrophoresis and polyacarylamide gel electrophoresis, but the latter is more advanced. The Saccharomyces cerevisiae strains ITS-1 sequences are most disalike, few of them are equal completely. The bigger differences are part of base pairs about Poly-T, others are exchanged by single base pair.

研究表明，以酵母菌株的rDNA ITS-1序列进行琼脂糖凝胶电泳，能把菌株分类鉴定到属的水平；采用非变性聚丙烯酰胺凝胶电泳能把部分酿酒酵母菌株分类鉴定到种的水平，区别效果较琼脂糖凝胶电泳有所提高；对rDNA ITS-1序列进行克隆、测序，经序列比对后发现，酿酒酵母属中酿酒酵母菌株的ITS-1序列大多数有差异，差异主要表现在ITS-1序列上游的一段Poly-T碱基上，其它处的差异仅是个别碱基的转换或颠换，ITS-1序列长度分布在360-370bp之间，G+C%含量均在35.3%左右。

A novel method for the measurement of insulin-link growth factor I with capillary zone electrophoresis was developed. The molecular interaction between IGF-I and its polyclonal antibody was primarily studied with immunocapillary electrophoresis and the binding constant was determined. The work shows the possibility of applying CE to immuno-analysis, which could be a new clinical analytical method for diagnosis, treatment and monitoring for the endocritic and metabolic diseases.

以分子间相互作用理论为依托，以胰岛素样生长因子-Ⅰ及其抗体为研究体系，建立了用毛细管区带电泳测定IGF-I的方法，并首次用免疫毛细管电泳方法研究了IGF-I与它的多克隆抗体的相互作用，测定了它们的结合常数，进一步证实了在毛细管电泳中实施免疫分析的可能性，为内分泌及代谢性疾病等威胁人类健康的常见病的临床诊断及治疗观察提供了新的检测方法。

CD was added into running buffer in above electrophoresis system, and the separation efficiency of polycarboxylic acids was improved further by the supramolecular interaction. Based on that, the method for separation and determination of several di- and tricarboxylic acids, including oxalic, malic, tartaric and citric acids, found in fruit juices was developed, which supplied a fast, convenient and sensitive method for the food analysis, the quality control of foods and various drinks.

果汁中多元有机酸的毛细管区带电泳/Cu电极安培检测在上述分析体系的电泳介质中添加β-CD，利用CPB与β-CD的协同超分子作用，进一步提高了多元有机酸的分离效率，成功实现了水果中四种常见多元有机酸草酸、柠檬酸、苹果酸、酒石酸的基线分离，建立了几种果汁中上述有机酸毛细管区带电泳/Cu电极安培检测定量测定方法，为食品分析、食品饮料质量控制以至辨别酒类商品真伪等提供了一种方便、快速、灵敏度高的分析方法。

A CEVd strain was isolated from Heibei province in China and its complete nucleotide sequences were identified.Accounting to the characteristic of structure of viroid, the nucleic acid from the positive material was detected by bidirectional PAGE.

柑桔裂皮类病毒快速检测技术体系的建立聚丙烯酰胺双向凝胶电泳法检测：将从阳性材料上提取的类病毒核酸，先在6％的聚丙烯酰胺非变性胶上进行第一向电泳，然后切割含类病毒分子的胶条，在变性条件下进行垂直或反向电泳，最后银染观察。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。