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After silver staining, the differential protein expression was analyzed by using PDQuest 2-D analysis software.

凝胶经银染色后用PDQUEST 6.2.1电泳分析软件对胸水中的蛋白质谱进行分析,识别差异表达的蛋白质。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA,在含有甲醛的琼脂糖凝胶电泳后,转印到硝酸纤维膜上。

With an effective length of 30 cm. The running buffer was a mixture of 25 mmol/L sodium borate and 100 mmol/L boric acid (pH 8.6). Effective mobilities of the two medicines changed with altering the concentration of β-cyclodextrin.

所用未涂层熔融石英毛细管的规格为内径50 μm,总长37 cm,有效长度30 cm,电泳缓冲液为25 mmol/L硼砂+100 mmol/L硼酸(pH 8.6),并加入不同浓度的β-CD。

We proposed, therefore, using adequate numbers of spawner obtai ned from various localities in artificial propagation and inoculating genetic ma terials from natural populations periodically are probably an effective measure to maintain or to increase the genetic variability in an artificially propagated fish stock.

我们认为生产上采用数量大、来源广的亲鱼进行人工繁殖,并定期用天然种群更换或补充繁殖用亲鱼很可能是保持或增加鱼类人工繁殖种群遗传变异性的一种有一效手段。关键词同工酶座位,电泳,遗传变异性,天然种群,草鱼

Research Progress of FSHβ Gene in Swine;2. The polymorphism of two loci FSHβ-1 and FSHβ-2 was detected by electrophoretic method and PCR-RFLP with re striction endonuclease HaeⅢ.

本研究以北京黑猪为研究对象,以 FSHβ亚基基因为产仔性状的候选基因,分别采用PCR产物直接电泳和PCR-RFLP方法来检测 FSHβ亚基基因2个位点的多态性。

The 2-DE profiles of days 1 and 4 were obtained through the method of IPG strip and about 350 spots were detected on each profile.

通过和五龄一天柞蚕丝腺蛋白的双向电泳图谱相比较发现:在五龄四天时蛋白表达有较大的差异。

The interactions of Strychnos alkaloids, fangchinoline,tetrandrine and carbamazepine with serum albumin were studied by fluorescencequenching spectra, synchronous fluorescence spectra, three-dimensional fluorescencespectra and ultra-v...

在系统查阅有关文献资料的基础上,进行了以下研究工作:(1)采用在线推扫预富集—胶束毛细管电泳法建立了测定中草药中马钱子生物碱的新方法。

RT-PCR was used to detect the expressions of COL1A1 mRNA in 20 patients with idiopathic congenital talipes equinovarus.

采用半定量RT-PCR方法检测20例单纯性马蹄内翻足患儿下肢肌肉及肌腱组织中COL1A1基因mRNA的表达,根据COL1A1基因转录调控区-1 031 bp~+30 bp及第1内含子的序列,设计8对引物, PCR扩增后,采用变性梯度凝胶电泳技术筛查突变并测序。

F neurons were observed in pigeon optic tectum slices using in vitro technique. Effect of glutamate and its antagonist glutamatic diethylester applied perfusedly and microiontophoretically on the discharge were analysed.

f亚层神经元的自发放电特征,以及灌流和微电泳给予谷氨酸(glutamate, Glu)及其受体拮抗剂谷氨酸二乙酯(glutamate diethylester, GDEE)对自发电活动的影响。

Form ternary phosphating film, fine grain size, special structure. Can adsorb thick cathodic electrophoretic coating, make film surface equality and high-brightness.

所形成的三元系磷化膜晶粒细密,结构特殊,可吸附较厚的阴极电泳涂膜,并使涂膜表面均匀完整光亮度高。

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