电泳的

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Chapter 4 gives a micellar electrokinetic chromatography method for the quantitative analysis of seven podophyllum lignans in podophyllum emodi wall.

第四章：建立了胶束电动毛细管色谱法分离测定抗癌药物桃儿七中七种主要有效成分的新方法；利用二阶导数电泳图定量测定低含量和在实际样品没有达到基线分离的组份。

Methods HIV-1 env gp41 gene of BH10 strain (nt6 977-7 497) was constructed into expressing vector pBV221 and expressed in E. Coli HB101. The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

表达产物通过15%SDS-聚丙烯酰胺凝胶电泳初步分离纯化，根据RF值，切下含特异蛋白的胶带，以Western blot法转移在硝酸纤维素膜上；免疫血清法检测合格者制备的抗原检测条带，经国家标准参比血清检测。

The present paper compared the major storage proteins in starchy endosperm of four autotetraploid rice lines with that of their corresponding diploid rice lines. The content of albumin, globulin, prolamin and glutelin in each sample was determined respectively.

以4份同源四倍体水稻和4份相应的二倍体水稻为研究材料，对其种子内清、球蛋白、醇溶蛋白和谷蛋白的含量进行了测定，利用聚丙烯酰胺凝胶电泳技术分析了其特异性。

The electrophoresis of the hemolysate of the propositus at pH 8.6 revealed that the abnormal hemoglobin was slow-moving and its mobility was similar to that of the G group.

先证者的溶血液在pH8.6电泳中出现泳速相当于Hb G组的慢速血红蛋白区带。肽链分析证实此异常血红蛋白为α链异常。

The course of theory teaches the principles and application of experiments, including Purification and clinical application of enzymes, Chromatography, Electrophoresis and Radioactive isotope application.

理论课讲授实验技术的原理及应用，包括：酶的纯化与临床应用，层析，电泳和放射性同位素应用简介。

Serum protein immunofixation electrophoresis can be valuable in diagnosis of MM for its rapidness and accuracy in determination.

血清蛋白免疫固定电泳技术能快速、准确地进行MM鉴定，对MM的诊断、病情判断、治疗及预后评估具有重要的临床价值。

Methods Three different resolvable solutions were adopted to take out the proteins of MOLT-4 cells as the example, and then the proteins were separated by two dimensional polyacrylamide gel electrophoresis.

以MOLT-4细胞为例，采用3种溶解性能不同的裂解液提取细胞中的蛋白质组，并分别进行双向聚丙烯酰胺凝胶电泳。

The SDS electrophoresis pattern of proteins in alfalfa plantlets showed that 54Kd, 28Kd and 17Kd proteins greatly synthesized in resistant plantlets. Tn sainfoin resistant plantlets, the synthesis of some proteins was also strengthened.

再生苗的可溶性蛋白质SDS电泳表明：54Kd，28Kd和17Kd的蛋白质在苜蓿抗性系再生苗中染色加强；23Kd和31Kd蛋白质在红豆草抗性系再生苗中染色较强。

METHODSThe venous blood with no sibship was taken from 261 cases (160 of male, 101 of female ) of KoreanChinese in Yanbian area, and the analysis of DXS7132 short tandem STR locus was performed by using PCR, polyacylamide gel electrophoresis and silver staining.

方法］选择261名(男性为160名，女性为101名)延边地区朝鲜族无血缘关系的个体静脉血，提取DNA，经PCR扩增、聚丙烯酰胺凝胶电泳及银染显色等，对朝鲜族人群DXS7132基因座的基因多态性进行分析。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。