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Column technology is one of the most important parts of capillary electrophoresis.

涂层柱的制备技术一直是毛细管电泳技术中的一个重要部分。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA,目的DNA的聚合酶链反应扩增及电泳检测,5-羟色胺2A受体基因T102C基因片断扩增的引物序列:5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3';采用50 μL反应体系,DNA 0.5 μg,引物50 pmol,TagDNA酶2U加dNTP至终浓度200μmol/L。

A cataphoresis discharge tube of 7mm inner diameter and 38cm active length was made for the He-Sr+ laser. The recombination laser at 430.5nm and the R-M transition laser at 1.03μm were obtained with the modified Blumlein circuit by high-frequency longitudinal pulsed discharge. The laser components are concentrated on the 430.5nm wavelength.

设计制作了7mm内径和38cm有效激励长度的电泳式He-Sr+激光管,采用修饰Blumlein电路,通过纵向高重复率脉冲放电激励,实现了一价锶离子复合激光430.5nm和R-M跃迁激光1.03μm的同时振荡,其中复合激光占主要成分。

Since the appearance of capillary electrophoresis , it has attracted huch attention from analytical researchers because of its celerity and high performance.

自从毛细管电泳问世以来,就以它的快速高效得到了广大分析工作者的青睐。

Whether respiration related nuclei such as nucleus retrofacialis might play a role in the mechanism of central chemoreception on respiraton or not was also studied using the methods of local HRP application, microinjection and microiontophoresis.

采用延髓腹外侧表面敷贴HRP、微量注射酸化人工CSF及微电泳H〓的方法,探讨了呼吸相关核团NRF是否也参与延髓腹外侧表面的化学感受功能。

High performance liquid chromatography was used to establish the fingerprint of ether extract of claret to distinguish claret quality stability.

采用高效毛细管电泳建立了干红葡萄酒的乙醚提取物的指纹图谱,并进行指纹图谱分析。

Secondly, quantifying markers: Because genes polymorphism or the codominant molecular marker electropherogram in a gene locus present 3 states, we can quantify them with -1,0 and 1 respectively.

将标记的在电泳时表现的三种状态:隐性纯合、杂合及显性纯合分别量化为"-1"、"0"和"1"。

Finally we observed the condensation between the polymer and plasmid DNA by the methods of agarose electrophoresis, fluorescence spectrometer and Multi-angle laser light scattering.

最后,利用琼脂糖凝胶电泳、荧光分光光度计以及多角度激光散射手段,对合成的多聚物与质粒DNA的缩合作用进行了检测研究。

SSCP was detected in exon 6 and exon9p of the gene, but not in the other exons.Results In polyacrylamide gel electrophoresis, We have not found abnormal conformer in both affected individuals and control cases. There were no differences in the DNA sequence between these two groups.

结果 在非变性聚丙烯酰氨凝胶电泳中,未发现DCM组和对照组该基因的外显子6和外显子9p出现异常构象,通过DNA测序证实两组的核苷酸序列没有差异。

A detergent used to denature proteins and cover them in uniform charge before gel electrophoresis.

一种在凝胶电泳前用来使蛋白质发生变性并用统一的电荷覆盖蛋白质的去污剂。

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