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Methods: For 4 tetrameric STR loci (D5S818, D8S1179, D18S51and D21S11) genetic research, monoplex PCR methods were established to study the allele frequency and the applicability of forensic medicine.

应用聚合酶链反应,聚丙烯酰胺凝胶电泳及银染分型技术,对延边地区109名朝鲜族无关个体进行群体调查,获得四种基因座的群体遗传学资料。

The effects of acetylcholine and atropine on the electric activities of neurons of nucleus parafascicularis thalami were observed in anesthetized rats with multimicropiptte technique and iontophoresis.

在30只大鼠上,用多管微电极细胞外记录和离子微电泳方法,观察了乙酰胆碱和阿托品对丘脑束旁核神经元电活动的影响。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例,分成三份,分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本,用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA,以RT-PCR方法扩增β-Actin基因,经1.5%琼脂糖凝胶电泳,溴化乙锭染色,紫外透射仪下观察分析。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态,比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增,扩增产物经琼脂糖凝胶电泳后分析结果。

A basically undegraded, partially degraded and totally degraded.

肌肉组织基因组DNA的凝胶电泳呈3种类型:基本无降解、部分降解、完全降解。

When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database.

我们利用双向凝胶电泳对小鼠卵巢蛋白进行了分离,在考马斯亮兰染色后将所有可见的蛋白点切取下,经胰蛋白酶消化以后用MALDI-TOF质谱仪进行鉴定。

A simple, fast, and reliable method based on capillary electrophoresis with electrochemical detection was developed for the separation and determination of rutin, ferulic acid, vanillic acid, chlorogenic acid, quercetin and caffeic acid in Radix Astragali and its medicinal preparations.

采用毛细管电泳-电化学检测法对中药黄芪的主要活性成分芦丁、阿魏酸、香草酸、绿原酸、槲皮素和咖啡酸进行了分离和测定。

Chapter three: A CE method to separate and determine the five active components, gastrodin, vanillyl alcohol, p-hydoxvhenzyl alcohol, \inllln and p-hydroxybenzyl aldehyde in Chinese herb Gastrvdiri Juiti Bl. and its preparations was developed.

第三章:建立了分析和分离中药天麻及其制剂中5种有效成分—天麻素、对羟基苯甲醇、香草醇、香草醛、对羟基苯甲醛—的毛细管区带电泳方法。

The results showed that a neural structural relation between the NRF and the ventrolateral medullary surface does exist.

结果表明NRF与腹外侧表面存在神经结构联系,微量注射酸化人工CSF和DLH于NRF能引起呼吸活动加强,微电泳H〓主要引起NRF吸气相关单位兴奋,呼气相关单位抑制,提示NRF在中枢化学感受功能中也起着一定的作用。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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