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In recent years, the popularity for the use of capillary electrophoresis in the pharmaceutical field has been accelarated by its simplicity, high efficiency and selectivity, large separatory capacity, and its relatively lower operational cost.

近年来,毛细管电泳因其具有较高的分离柱效、选择性和分离能力以及快速、进样量小、操作简便并易于实现自动化等特点在药物分析方面倍受青睐。

In chapter 3, two on-line concentration CE methods for determination of sialic acid in saliva were developed.

在第三章,我们建立了两种在线富集毛细管电泳法测定人体唾液中的唾液酸。

A method for the determination of sialic acids in serum with ultrafiltration-capillary electrophoresis is described and the operation was optimized.

建立了用超滤-毛细管电泳技术测定血清中唾液酸含量的方法,并考察其最佳操作条件。

Methods Four pairs of these primers that would be amplified in simulium COI gene were designed by Primer Premier 5.0, the results of PCR would be investigated through agarose gel electrophoresis.

运用Primer Premier 5.0软件设计4对蚋科COI基因的PCR扩增引物,通过PCR扩增后进行琼脂糖凝胶电泳检测实验。

The differential expression protein with MALDITOFTOF by means of mass spectrographic was analyzed.

结果 重复性好的双向电泳图谱在CA 125阴性卵巢上皮性恶性肿瘤患者血清蛋白质表达谱发生了改变。

Two-dimensional gel electrophoresis were employed to analyze the proteome from leaves of cucumber R17 at 24, 48 and 72 h, after inoculating Sphaerotheca fuliginea .

以黄瓜抗白粉病品系R17为试材,在接种白粉病菌24、48和72 h后提取叶片蛋白质,采用双向电泳技术研究白粉病菌胁迫条件下黄瓜叶片蛋白质组的变化。

Spreng was much more simple and efficient than the conventional methods. And the extracted DNA was suitable for the polymorphic analysis based on PCR, such as ISSR-PCR.

通过琼脂糖凝胶电泳和OD260/OD280比值测定,所得基因组DNA纯度高,可作为ISSR等以PCR为基础的DNA多态性标记。

This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation.

本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。

Objective To establish a new method for isolating serum β-lipoprotein subfraction s using second double gradient polyacrylamide gel electrophoresis.

目的建立一种分离血清β脂蛋白亚组分的新方法———二阶双梯度聚丙烯酰胺凝胶电泳法。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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