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The Esterase, Catalase, PPO isoenzyme and soluble mycoprotein patterns of 43 isolates were analyzed by polyacrylamide gel electrophoresis.

利用聚丙烯酰胺凝胶电泳技术对43个新月弯孢分离物进行了酯酶等三种同工酶及可溶性蛋白的分析。

Objective To develop appropriate conditions for profiling cell proteome of Myxococcus xanthus.

目的:建立可以对黏细菌蛋白质组进行阵列分离的双向电泳条件。

Optimal conditions of 2-DE for proteomic analysis of Myxococcus xanthus has been established, which paves the way for global profiling of protein expression patterns during the development of the model organism.

初步建立了黏细菌总蛋白质组的双向电泳条件,为在细胞水平分析该模式生物发育时期蛋白表达谱奠定了基础。

Methods Total cellular proteins from Myxococcus xanthus DK1622 negetative cells were extracted and subjected to two-dimensional gel electrophoresis under different conditions.

以黏细菌模式菌株DK1622为材料,不同方法制备DK1622在管养性生长阶段的蛋白质组样品,双向电泳阵列菌体总蛋白。

And many new measures have appeared showing NACE can provide some unique advantages in separation methods.

但普通毛细管电泳一般是在水相中进行,这就要求分析物要有一定的亲水性。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果:细胞接触肽段后存活率下降28.9%;光镜下可见细胞贴壁不良,胞体缩小,细胞突起断裂缩短;荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞;电镜下可见胞质中出现空泡样结构,细胞染色质浓集于核膜内侧并裂解成碎块状;流式细胞仪检测细胞出现亚二倍体峰,DNA电泳出现梯状带。

The dissertation consists of six chapters: Chapter 1 provides a review on the mechanism, the different method and application of nonaqueous capillary electrophoresis.

首次用毛细管电泳方法分离和测定了某些藏药中几类活性成分,并建立了鉴别龙胆科藏药和中药的新方法; 3。

Methods After ZTO injection acted on human ovarian cancer SKOV3 cells, the changes in the nucleus were observed by fluorescence microscope, oncosis index was counted by projection electron microscope, the situation of cell DNA breakage was observed by agarose gel electrophoresis, and cell Bcl-2 gene expression was observed by immunohistochemical method.

以不同浓度莪术油注射液作用人卵巢癌SKOV3细胞后,通过荧光显微镜观察细胞核变化、透射电镜计算细胞胀亡指数、琼脂糖凝胶电泳观察细胞DNA断裂情况、免疫组化观察细胞Bcl-2基因的表达。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落,提取重组质粒,经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定,成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23~244)]和pMYO2[Ov rPrP(91~244)]。

Rice aging leads to the decreasing of total starch content and Fla content of amylopection, strengthen the rice starch crystallite and tightening the organization framework of endosperm, which was considered as the main cause leading to the changing rice starch gelatinization properties, rice aging leads to some degree of cross-link of rice oryzenin, SDS-poly-acrylamide gel electrophroresis gave the same result.

陈化导致米谷蛋白的交联,SDS聚丙烯酰胺凝胶电泳验证了这一结果。

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