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Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Sodium lauryl sulphate-polyacrylamide gel electrophoresis method was used to detect the molecular weight of LiBr dissolved fibroin solution.

采用十二烷基硫酸钠-聚丙稀酰胺凝胶电泳法测定溴化锂溶解后丝素溶液的分子量。

Based on the sources, molecular weights, pI, characters of chromatography and electrophoresis, substrate specificities of these enzymes, it can be concluded that many kinds of nuclease-like enzymes have been found in the tissues of Lumbricus Bimastus.

根据这些酶的来源、分子量、等电点、层析和电泳等行为以及底物特异性等,初步证明,我们新近发现了双胸蚓组织中存在着多种核酸酶样酶。

The results indicated that DNase E belonging to endonuclease.Conclusion1. Established a preparation technics of rude extracts of Lumbricus Bimastus nucleases.

应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定,证明了DNase E属于核酸内切酶。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析,8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达,表达蛋白分子量为140~150kDa,抗原cDNA基因经限制性内切酶EcoRI酶解后,琼脂糖凝胶电泳显示其大小为700~900bp,PCR能扩增出特异性条带。

Using two-dimensional gel electrophoresis, the protein of whole mantle of patterned and non-patterned Meretrix meretrix , from Yueqing, Zhejiang, East China, was studied in mass spectrometry.

中文摘要:采用双向电泳技术,对浙江乐清无花纹和有花纹文蛤外套膜的全蛋白进行了研究,并利用质谱和软件对差异蛋白进行了分析。

Ten isozymes and proteins of Microtus forth were investigated by cellulose acetate membrane electrophoresis.

应用乙酸纤维素膜电泳对四类东方田鼠的同工酶与异构蛋白生化基因位点进行测定分析。

With the method of multitube electrode recording, the transmitter characteristics of LA inhibiting the acoustic response of neurons in A-Ⅰ has been studied.

七运用多管微电极记录方法结合微电泳技术,探讨了LA抑制皮层AⅠ神经元声反应的递质特性。

To establish a method for HPCE finger printing determination of white muscardine silkworm.

采用高效毛细管电泳法建立白僵蚕的指纹图谱。

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