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Penicillin V acylase from the crude culture filtrate of Fusarium oxysporum FP 941 was purified with a single band on non-denaturing electrophoresis by means of (NH4)2SO4 fractional precipitation, adsorption on, and elution from, celite, ion-exchange column chromatography with DEAE-cellulose, and Sephadex G-200 gel filtration chromatography.

通过硫酸铵沉淀、硅藻土吸附、DEAD-纤维素离子交换层析和Sephadex G-200凝胶过滤,由尖镰孢FP941培养滤液中得到了聚丙烯酰胺凝胶电泳均一的青霉素V酰化酶。

Methods: Biological technique, including centrifugalization, ion-exchange chromatography, hydrophobic chromatography and dialysis was used. Osteopontin from bovine milk were identified by SDS-PAGE and Western Blot.

运用生物化学技术,通过离心脱脂后,再经过离子交换层析、疏水层析、透析等技术达到分离纯化目的;随后对分离纯化得到的产物进行SDS-PAGE电泳、免疫印迹技术鉴定。

Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDS PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum.

结果: 藜草花粉经电泳后得到12条蛋白质区带,分子质量依次为92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4和18.5~12 ku;免疫印迹可见4条sIgE反应带,蛋白分子质量依次为92, 34, 31.6和18.5~12 ku;与患者血清反应率分别是70%, 50%, 80%和90%;与血清sIgG结合的反应带有8条,蛋白分子质量为92, 61, 52, 45, 43, 39.7, 31.6和18.5~12 ku;与患者血清反应率分别是80%, 40%, 10%, 20%, 80%, 10%, 10%和100%。

Casein from the same individual during the colostral period differed in mobility as showed by alkaline urea-PAGE analysis.

牦牛初乳酪蛋白碱性-尿素PAGE分析显示,同一个体β-酪蛋白的电泳迁移率在初乳期有差异。

UV-B radiation during the cell cycle induced accumulation of cyclobutane pyrimidine dimers.

单细胞凝胶电泳检测结果表明,UV-B辐射引起拟南芥根尖细胞内积累大量的环丁烷嘧啶二聚体。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状。

The genome DNA was amplied and electrophoresis of Sichuan 7 native goat breeds and1 cultivated breed, including Tibet goat(n=27)、ChengDu Ma goat(n=20)、JinTang blackgoat(n=21)、LeZhi black goat(n=24)、JianChang black goat(n=23)、BeiChuan white goat(n=26),Jian Yang Daer goat(n=30) and NanJiang yellow goat (n=90), using 5 pairs of microsatelliteprimers.

选用5对微卫星引物,对四川7个地方山羊品种—简阳大耳羊(n=30)、建昌黑山羊(n=23)、成都麻羊(n=20)、藏山羊(n=27)、金堂黑山羊(n=21)、乐至黑山羊(n=24)和北川白山羊(n=26)及1个培育品种—南江黄羊(n=90)的基因组DNA进行PCR扩增,并进行电泳分析。

Objective To explore the dosage effects of low power laser irradiation on erythrocyte deformability and electrophoretic mobility .

目的探讨低强度激光辐照下其照射剂量与红细胞变形指数、红细胞电泳率改善效应之间的关系。

The molecular weight and distribution of samples were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳方法确定3种样品的相对分子质量及其分布。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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