电泳的

Leukocyte proteins concentrations were determined with Bradford Method. Phosphoproteins in the collected leukocyte proteins were enriched by BDTM Phosphoprotein Enrichment Kit. Finally the enriched phosphoproteins were separated by SDS-PAGE and dyed by argentation, and the electrophoresis map of SDS-PAGE was analyzed by Scion Image Tool.

刺激组加入表皮生长因子(40 μg/L)；抑制剂组加入细胞外信号调节激酶特异性抑止剂UO126 （10 μg/L）和表皮生长因子（ 40 μg/L）；对照组加入等量的培养基，用Bradford法测定细胞蛋白质含量，BDTM Phophoprotein Enrichment Kit分离富集白细胞磷酸化蛋白质，SDS-PAGE分离磷酸化蛋白质，银染法染色，采用Scion Image软件分析电泳结果。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Objective: To establish two-dimensional electrophoresis(2-DE) patterns of flower-bud for Betula platyphylla.

目的：初步建立白桦花芽蛋白质组研究的双向电泳(2-DE)技术，以提高其分辨率及重复性。

MethodsHigh-performance capillary electrophoresis was used to analyze the chemical composition of Brassicaceae.

方法利用高效毛细管电泳法分析十字花科植物中化学成分（主要是以黄酮类成分为依据）的差异。

ResultsThe electrophoregrams of the studied species had more or less difference, so it could determine the relationship of different species in Brassicaceae.

结果所研究材料不同种属间电泳图谱存在或多或少差异，可以初步判断其亲缘关系的远近。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变；沉淀回收的PCR产物以20μl变性上样液（95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝）溶解后热变性为两条DNA单链后，上样于8%非变性聚丙烯酰胺凝胶（含5%甘油），700伏电压、4℃电泳16小时。

By Using SDS-PAGE gel electrophoresis inorder to study the expression of crystalline in lens regeneration of Bufo Raddei Strauch Tadpole.

用SDS-PAGE电泳方法研究再生过程中晶状体蛋白的表达。

The result of protein concentration analysis, activity detection and sodium dodecyl sulphate - polyacrylamide gel electrophoresis demonstnated that protein content of IgG purified by ammonium sulfate is higher than that of caprylic acid - ammonium sulfate, but the purity and activity of IgG purified by caprylic acid - ammonium sulfate is far higher than that of ammonium sulfate.

本研究中对辛酸-硫酸铵法和硫酸铵法提取鸭血清中IgG进行了比较，经蛋白含量测定和SDS-PAGE电泳分析表明，虽然硫酸铵法提取蛋白含量要比辛酸-硫酸铵法高，但辛酸-硫酸铵法提取IgG的纯度和活性都明显高于硫酸铵法。

Hapten-carrier protein conjugates were made using ciprofloxacin and two carrier proteins by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride method.

采用非变性凝胶电泳法和紫外分光光度法对偶联物中环丙沙星与载体蛋白的分子结合比进行分析。

The carriage bottom paint both use the cathodal e-coating paint, which can effectually improve the fastness of the paint's attachment.

油漆工艺：12道涂装工序、多道预处理工艺，车身底漆全部采用阴极电泳底漆，有效提高油漆附着牢度，加上严格的油漆工艺规程，确保车身外观常年光亮如新。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。