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METHODSThe venous blood with no sibship was taken from 294 cases (187 of male, 107 of female) of KoreanChinese nationality.The DXS9898 short tandem STR locus was performed by using the techniques of PCR, polyacylamide gel electrophoresis and silver staining.

方法]随机抽取294名延边地区无血缘关系的朝鲜族个体,其中男性为187名,女性为107名,采集静脉血,提取DNA,行PCR扩增,变性聚丙烯酰胺凝胶电泳及银染显色,行遗传基因多态性分析。

The gels were stained with silver, and the differential protein expressions were analyzed with Melanine 3 software. Results The protein spots of the two cell lines were 1285±51 and 1184±47 (SW480) respectively, containing 96±7 differential protein spots with 50±6 expressed or obviously increased only in LST cell line and 47±5 in SW480 cell line.

结果 LST和SW480细胞株双向电泳图谱蛋白质点数分别为1285±51和1184±47,共获得96±7的蛋白质差异点,其中50±6个点仅在LST细胞株中表达或表达明显增强,47±5个点仅在SW80细胞中表达或表达明显增强。

METHODS The coimmunoprecipitation, sodium dodecyl sulfate polyacry amide gel electrophoresis, peptide mass fingerprint and Western blot analysis were used to isolate and identify the interacting proteins of DNA-PKcs.

通过免疫共沉淀获得DNA-PKcs的相互结合蛋白复合物,SDS-PAGE电泳分离蛋白,肽质指纹分析和Western印迹鉴定蛋白。

In chapter 6, a method for separation and determination of Cl〓, NO〓 and SO〓 in river water by CE with indirect UV detection was described. Background electrolyte consisted of sodium borate, chromate, and CTAB was employed for the separation.

在第六章,采用毛细管电泳间接紫外法在硼砂-铬酸根-CTAB体系中分离与测定了河水中的Cl〓,NO〓和SO〓。

The optimal separation for capillary electrophoresis is 20% methanol, and 0.13mM of β-cyclodextrin in 25mM sodium tetraborate buffer solution(pH 9.1), and 20℃of temperature.

实验结果显示最佳化的毛细管电泳分离条件为25mM四硼酸钠溶液(pH= 9.1),添加0.13mM β-cyclodextrin 及20%甲醇溶液。

Main results shown as follows:Three methods—SDS method, simple CTAB method and improved CTAB method were used to extract genomic DNA from sporophyte of Laminaria.

主要结果如下:对分别用SDS法、常规CTAB法、改良CTAB法三种方法提取的海带基因组DNA,进行紫外吸收检测、电泳检测和RAPD扩增检测。

Processing characteristics of soybean have been found to have a close relationship with the subunit composition of soy protein.

本研究利用SDS-PAGE电泳分析大豆蛋白质组成并提出了便捷的样品预处理方法。

E. sulfadiazine, sulfamerazine and sulfadimidine (SM2), by capillary zone electrophoresis with conductivity detection was developed.

采用毛细管电泳-电导法建立了磺胺嘧啶、磺胺甲嘧啶、磺胺二甲嘧啶3种结构相近磺胺药物的分离检测方法。

Thermophile was purifided to homogeneity, by using ammonium sulfate fraction, DEAE-Sepharose chromatography, Phenyl-Sepharose chromatography and Sephacryl S-100 chromatography.

粗酶液经硫酸铵分级沉淀、DEAE-Sepharose阴离子层析、Phenyl-Sepharose疏水层析、SephacrylS-100分子筛层析等步骤获得凝胶电泳均一的内切β-葡聚糖酶。

The distributions of aminoacylase and alkaline phosphatase had been studied in the common seaweeds in Dalian area, and the Ulva pertusa Kjellm had been chosen as the target for isolation and purification. These two enzymes had been purified for a single band in the SDS-PAGE and their basic characteristics had been studied, too.

研究了氨基酰化酶和碱性磷酸酶在大连地区常见海藻中的分布状况,从中筛选出孔石莼作为纯化研究对象,并从孔石莼中分离纯化出了这两种酶,纯度达到了聚丙烯酰胺凝胶电泳一条带。

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