电泳的

Result The background noise of bidirectional electrophoresis map of protein extracted by TCAJA method was greater, its clarity was poor, its horizontal striations were more and there were tinny spots with irregular shape appeared on it. The variegated spots had difference from the normal protein spots, they formed high coverage and shading on the other protein spots and affected the accurate expression of map information. The background noise of bidirectional electrophoresis map of protein extracted by Trizol method was little, its horizontal striations and non-protein impurities were less, there was no shading phenomenon strong spots and mere protein spots, so it provided abundant information for quantitative and qualitative analyses on the proteome from buffalo grass seeds, Compared with TCA/A precipitation.

结果］TCA/A法提取的蛋白质双向电泳图谱背景噪音大，清晰度差，横向条纹较多，图谱上呈现多个形状不规则的斑点，杂色与正常蛋白质点有区别，对其他蛋白质点构成高度及盖和遮进，影响图谱信息的准确表达；Trizol法所提取的蛋白质样品，其双向电泳图谱背景嗓音小，横向条纹少，非蛋白类杂质少，无斑点间的这进现象，蛋白质点数量增多，为野牛草种于蛋白质组定量、定性分析提供了丰富的信息。

In the process of electro-membrane filtration, the applied operation condition and membrane do not have significant electroosmosis effect through related experiment. Under the operation of 98KPa pressure, only 6% flux was increased, which showed the increase of flux in the applied electric field process mainly from electrophoresis effect. The effect of dye filtration by membrane is mostly influenced by the basic nature of dye, including size of molecular weight, electrophoresis motility and the adsorbability of dye to membrane.

在外加电场薄膜程序中，所用的操作条件以及所使用的薄膜，经电渗透实验显示电渗透的效应不明显，而在98KPa压力操作下，也仅增加6%的通量贡献，显示外加电场程序通量之提升主要来自电泳作用；薄膜过滤染料的效果主要受到染料基本性质的影响，包括分子量大小、电泳动能力与染料对薄膜的吸附能力。

Chapter 4 Capillary Zone Electrophoresis with Amperometric Detection forComposition Analysis of LaminarinThe composition of laminarin was firstly determined by analyzing its hydrolysis monosaccharides with capillary zone electrophoresis - amperometric detection.

第四章毛细管电泳一安培检测法测定昆布中多糖成分本论文首次用毛细管电泳一安培检测法测定了昆布多糖水解单糖的成分，实现对(来源：Aa4BC9b2b论文网www.abclunwen.com)昆布多糖的测定。在选定的最佳条件下，昆布多糖水解产物岩藻糖、半乳糖、葡萄糖、甘露糖、木糖在20min内实现完全分离而且在铜电极上有着很好的电流响应。通过对昆布多糖中水解单糖成分含量的计算显示该多糖中岩藻糖、半乳糖、葡萄糖、甘露糖、木糖的摩尔比为

At the web-based control page, it is able to upload our 2-D PAGE data and download the Tag Image File Format image. The 2-D PAGE data can be searched easily by limitation such as Peptide, NCBI Accession number, Map, Organisms, pI/Mw range, Drug/Compound, and Operator.

在这个网路管理介面内，我们可上传二维聚丙烯醯胺胶体的电泳资料、下载标志图形档案格式的影像图片，并且我们可以很容易的藉由如：蛋白质的名称、NCBI的登录号码、图像、胶片所使用的物种、蛋白质的等电点值与分子量范围（pI/Mw range）、胶片使用的抗生素或化合物（Drug/Compound）以及实验操作者等条件来搜寻二维聚丙烯醯胺胶体电泳资讯。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Combined with high sensitive detective method, such as laser-induced fluorescence, electrochemistry, chemiluminescence and mass spectrometry, microfluid chip and capillary electrophoresis is becoming one of important and useful tool in post-genome era including proteomics as the results of theirs high performance, high speed and high throughput.

在生命科学的研究中，以毛细管电泳、微流控芯片毛细管电泳为代表的高速、高效、高通量分离分析技术与以质谱、激光诱导荧光为代表的高灵敏度、高选择性检测技术的联用，已成为后基因组时代的主流技术，是最重要的研究工具之一。

Seven biogenic amines were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol （95∶5, v/v）was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges.

样品经6%过氯酸萃取后，由苯甲酰氯衍生化，以含0.06 mol/L脱氧胆酸钠的0.02 mol/L硼酸(pH 9.2)-甲醇(体积比为95∶5)混合液为电泳介质，电泳电压25 kV，温度25 ℃，检测波长214 nm，在12 min内实现了7种生物胺的完全分离。7种生物胺的浓度与其峰面积在一定的范围呈良好的线性关系，检出限除组胺为15 μg/g外，其余均为5 μg/g。

To connect a capillary with an electrophoresis chip, a dead volume was usually created due to a hole with a conical-shaped bottom formed by drilling into the edge of the chip after the capillary was inserted into the hole.

为了丰富电泳芯片的功能及扩大其应用范围，芯片与外界的液体连接问题现在正成为一个新的研究热点[1～4]，芯片与传统的毛细管电泳检测器的连接是其中一个主要的研究方向[1～3]。

No US-pin plastic treatment therapy, a comprehensive alternative to traditional plastic injection of the United States (with the needle and micro-needle) is efficient, non-invasive and safe,"mesoderm into beauty therapy" is the latest technology of water use and water channel protein electrophoresis principle of development Note the infiltration hole electric technology to non-needle injection principle, the use of "point to a plane," AMD permeation technology in skin electroporation Act under the influence of pulsed current stimulation, so that pores have a physical reaction to the rapid spread in two seconds 650-800 of fine pores, forming an instant electronic delivery channels of nutrition, nutrition Note dialysis therapy immediately with electrophoresis, soft laser stimulation and ice, etc., in the most secure and fast, the skin will be required for nutritional products , 99% transmitted to the basal cells, and can be stored as long as 72 hours, the skin smooth and more compact, creating a perfect Beautiful Skin.

无针美塑治疗疗法，是全面替代传统注射美塑的高效、无创，安全的&中胚层导入美容疗法&，是运用最新技术水电泳和水通道蛋白原理研制出的电孔注渗技术，以无针注射原理，采用&点到面&超微渗透技术，其电穿孔法令肌肤在脉冲电流刺激影响下，使毛孔产生物理反应，在两秒内迅速张开650-800个微细毛孔，瞬间形成电子营养输送通道，营养注渗疗法随即展开，配合电泳、柔激光和冰封等刺激作用，在最安全和快速的情况下，将皮肤所需的营养产品， 99%输送到基底层细胞，并可储存72小时之久，令肌肤更紧致柔滑，缔造无瑕美肌。

MATLAB is a special-purpose computer program optimized to perform engineering and scientific analysis.It's easy to use and have a lot of powerful toolboxes which can be used in many aspects of engineering and scientific researches.

摘 要：凝胶电泳是当今分子生物学的一项最基本的试验技术，它的原理十分简单，但是通常只能通过目测marker的位置或是计算电泳率来得到样品的分子量，这样得到的结果是十分粗糙的。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。