电泳的

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

The content ofone liver protein band (R_f=0.79) at 1℃was significantly higher than at room temperature (P＜0.05).On the 2-D native-PAGE/SDS-PAGE gel, the content of P spot (MW=51kDa, R_1=0.47on the 1D native PAGE gel) at 1℃was significantly higher than at room temperature (P＜0.05), and the content of N spot (MW=65 kDa, R_f=0,94 on the 1D native PAGE gel) at roomtemperature was significantly higher than at 1℃. These protein spots may be related with the adaptation to the low temperature of the Heilongjiang wood frog.

黑龙江林蛙肝脏蛋白电泳显示，胶浓度6％的肝脏PAGE图谱分辨率高、谱带多，可分辨出15～19条谱带，其中1℃组R_f=0.79蛋白含量比室温组显著增加(P＜0.05)。2-D native-PAGE／SDS-PAGE电泳时，胶浓度为12％时分离效果较好，蛋白斑点较多。1℃组中P蛋白斑点(MW=51kDa，一向PAGE的R_f=0.47)显著性上调(P＜0.05)，N蛋白斑点(MW=65kDa，一向PAGE的R_f=0.94)显著性下调(P＜0.05)，推测这两种蛋白可能与黑龙江林蛙在低温环境下的适应性变化有关。

In order to explore the extraction method of proteome and technique of 2-DE, we compared the extraction method between the TCA-ac-tone and the Tris-HCl, and improved the key steps of 2-DE. The Tris-HCl extraction was the most appropriate for Antiaris toxicaria seed proteome analysis because of the highest resolution and more informative 2-DE map. Those were the solid basis for the later research of studying on the discrepancy expression proteomics of Antiaris toxicaria Seed.

在探索适合箭毒木种子蛋白质组学研究双向电泳方法中，比较了三氯乙酸－丙酮沉淀法、和Tris-HCl法，以及对双向电泳过程中的关键步骤的改良，认为Tris-HCl法为最适方法，所得图谱背景清晰，蛋白质信息量最大，为箭毒木属植物的差异蛋白质组学的后续研究打下了坚实的基础。

Among the various electrophoresis technologies, the electrophoresis with a supporting media of polyacrylamide gel has become a common separate analysis method in lab and other fields, such as biochemistry research and clinical test, due to its steady chemical properties, excellent repeatability, small electroendosmosis, easy operation and high sharpness of separation.

在众多的电泳方法中，以高分子材料—聚丙烯酰胺凝胶为支持介质的电泳因其化学性质稳定、重复性好、电内渗小、操作简单及分辨率高等优点，已成为实验室常用的分离分析手段，在生化研究和临床检验等领域日益显示出其强大的生命力。

By the analysis of SDS-PAGE electrophoresis, CXJZ95-198 secrets exocellular proteins bands in media of glucose, mannose ,xylan, mannosan and pectin respective were 32 bands, 33 bands,35 bands, 36 bands and 21 bands respectively .

在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中的发酵液在SDS-PAGE电泳中分别分泌出32条，33条，35条，36条和21条蛋白带；多数可比较的蛋白质电泳谱带的颜色深浅呈魔芋粉＞甘露糖＞木聚糖＞葡萄糖＞果胶的趋势。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点，发酵液经离心，丙酮沉淀，Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品，比活力较原酶液提高了9.8倍，回收率达到了32.98%，用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

Process design of cathodic electro- phoresis coating line for middle or small work pieces such as shell s and windscreen wipers was discussed in particularly.

详细论述了炮弹、汽车雨刮器等中小工件阴极电泳涂装生产线的工艺设计，阐述了生产线的基本型式、设备组成和设计原则，介绍了一种新型的针对中小工件阴极电泳涂装的步进直线式T型行车输送涂装生产

The four carbamates were hydrolyzed in alkalescent aqueous solutions, resulting in the formation of 2-sec-butylphenol, 2-isopropylphenol, m-cresol and α-naphthol, which could be determined by amperometry after capillary electrophoretic separation.

用毛细管电泳-安培检测法测得酚类化合物的量，然后分别换算为等当量的氨基甲酸酯类农药的量，从而建立了用毛细管电泳-安培检测法检测所选四种农药残留的方法。

Results Well-resolved, reproducible 2-DE profiles of human colon carcinoma tissues and paired normal tumor-adjacent colon tissues were obtained. For colon carcinoma tissues, a total of (980±28) spots were detected; for paired normal tumor-adjacent colon tissues,(860±45) spots were detected. For normal tumor- adjacent colon tissues, the average deviation of spot position was (10.487±0.11) mm in IEF direction and (0.944±0.12) mm in SDS-PGE direction; for colon carcinoma tissues the average deviation of spot position was (0.654±0.11) mm in IEF direction and (1.20±0.22) mm in SDS-PGE direction. Number of differentially expressed proteins was 72.00±12.34 between colon carcinoma and paired normal tumor-adjacent colon tissues.

结果 建立了结肠癌及正常肠组织的双向凝胶电泳图谱，其中癌旁正常结肠组织和结肠癌组织电泳图谱中平均蛋白质点数分别为860±45和980±28；癌旁正常结肠组织和结肠癌组在IEF方向上的平均偏差分别为(0.487±0.11)mm和(0.654±11)mm，在SDS-PAGE方向上的平均偏差分别为(0.944±0.12)mm和(1.20±0.22)mm，结肠癌与癌旁正常结肠组织的差异表达蛋白质点数为72.00±12.34。

Composition of low molecular weight glutenin subunits and gliadins were investigated with thirty common wheat cultivars which are widely grown in Shangdong province. In our study we used acid polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis, analysed Sedimentation test, Protein content, Mixing time, Stability, Max-resistance to extension, Farinograph Quality Number and Extensogram area of the 30 common wheats. They have close relationship with the technology properties of noodle wheat cultivars.

本研究通过酸性聚丙烯酰胺凝胶电泳和SDS-聚丙烯酰胺凝胶电泳，分析了30个山东省种植面积较大且具有部分代表性的普通小麦品种的低分子量麦谷蛋白亚基和醇溶蛋白组成，测定了30个品种的蛋白质含量、沉淀值、面团形成时间、稳定性、粉质评价值、最大拉伸阻力和拉伸曲线面积等面粉及面团主要品质性状，分析了低分子量谷蛋白亚基和醇溶蛋白组成与面条小麦品质性状的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。