电泳的

Chapter one: it is an introduction of the basic theory of capillary electrophoresis and on-line concentration techniques.

第一章：介绍了毛细管电泳的基础理论和毛细管电泳在线富集技术的发展状况。

Using progesterone and bovine serum albumin as model compounds respectively, fluorescent labeled small molecule hapten and large molecule antigen were prepared.

1以孕酮和牛血清白蛋白为模型化合物，制备了小分子半抗原和大分子抗原的荧光标记物，研究了标记物的抗原活性和光谱性质，结果表明标记物是有效的毛细管电泳免疫分析试剂；制备了抗体F〓片段，研究了其抗体活性，结果表明F〓片段比整个抗体更适合作为毛细管电泳的免疫分析试剂。

The results showed that the total dissoluble proteins hands of 8% and 10% gel were 91 and 97, respectively, and the seed salt soluble proteins bands of 10% gel were 69; All of the five alfalfa species have their own characteristic bands, which are different in number, distribution, width, and concentration; Among three methods, the five species' seed dissoluble protein fingerprints of 10% gel' bands is relatively more, which genetic diversity scattered in A, B and C regions, and can be repeated in different experiments. So it's the best fingerprint. The fingerprints of the seed dissoluble proteins and seed salt soluble proteins of five species are unanimous, which showed that: the hybrid has the nearest genetic distance with Medico go sativa cv.

结果显示：5/个种10%和8%凝胶电泳的种子可溶性蛋白总带数分别为97和91， 10%凝胶电泳的种子盐溶蛋白共有69条谱带；5种苜蓿在种子可溶性蛋白和盐溶蛋白电泳图上都具有各自的特征谱带，谱带数目、位置、宽窄和颜色深浅都存在明显的差异；10%凝胶的可溶性蛋白指纹图谱谱带数目相对较多，种间遗传差异在A、B、C区均有分布，谱带的重复性好，是鉴别种的最好图谱。5种苜蓿种子可溶性蛋白和盐溶蛋白指纹图谱一致，表明杂交种和敖汉苜蓿的谱带相似性最大。

The sensitivity of capillary electrophoresis was about 750 times higher than that of the agarose gel electrophoresis, which can only detect the 1/3 of the total PCR product of 74 picogram of template DNA (as amount of DNA in 13 cells).

毛细管电泳检测微量目的基因扩增产物的综合灵敏度是琼脂糖凝胶电泳的750倍。

Firstly, the Protein compositions of various parts of the spermatophore were studied through the methods of fractionation and comparing PAGE. And a method for spermatophore fractionation was established, the feasibility of this method was examined by the means of PAGE and transmission electronic microscopy.

首先，通过分级分离和比较电泳的方法对中华绒螯蟹精荚各部分的蛋白组分进行分析，并在试验过程中，建立起一套精荚分级分离的方法，通过电泳分析和透射电镜观察等手段对这一方法的可行性进行了验证。

The appearance of capillary electrophoresis is a revolution in traditional electrophoretic technique.

毛细管电泳的出现是对传统电泳技术的一次革命，它结合了电泳的高效分离原理和色谱的毛细管柱制备方法与自动化操作技术，成为当。。。

Finally, preparative separation of proteins by nonaqueous electrophoresis operated in MFE was attempted.

最后采用多通道流动电泳技术进行了非水介质制备电泳的研究。

By use of organic solvents as separation media, nonaqueous capillary electrophoresis, which expands the application of CE, has some merits that can't be found in conventional CE.

非水毛细管电泳由于使用了有机溶剂作为分离介质，具有一些常规毛细管电泳所不具备的优势，拓宽了毛细管电泳的应用范围，在分析水不溶性物质方面具有独到的优势。

The history of CE was retrospected and the basic theory of CE was introduced briefly.

对毛细管电泳的发展历史做了简单的回顾，同时对毛细管电泳的一些基本概念和原理做了简单的介绍。

In this dissertation, our interests are focused on the study of capillary zone electrophoretic analysis of traditional Chinese medicines through optimizing experimental parameters which are of key importance for CE performance.

我们的工作便是在毛细管区带电泳的理论指导下，通过优化选择影响分离性能的各种实验参数，将毛细管区带电泳用于具有研究和应用前景的中药领域，提出了一些新方法。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。