电子显微镜

By light and electron microscopy, there were many morphological characteristics of apoptosis including compaction and margination of nuclear chromatin, nuclear fragments, and apoptotic bodies.

光学显微镜和透射电子显微镜可观察到细胞凋亡的形态学改变。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

Paraffin section technique was applied in the study of the development of the seed coat of Pinus kwangtungensis. The results showed that the development of seed coat follows with the development of the megaspore mother cell. In the development of female gametophyte, a thin, dense and dark layer of cells formats around the female gametophyte.

运用石蜡切片法，在透射电子显微镜和光学显微镜下观察华南五针松种皮的形态结构和发育，用PAS反应显示细胞内淀粉粒分布，研究细胞内淀粉粒的含量。

Metallography is the study of the microstructure of metals and alloys by means of the metallurgical microscope and the electron microscope .

金相学是用金相显微镜和电子显微镜对金属和合金的显微结构的研究。

Metallography is the study of the microstructure of metals and alloys by means of the metallurgical microscope and the electron microscope.

金相学是用金相显微镜和电子显微镜对金属和合金的显微结构的研究。他们采取一切羞辱手段来摧毁犹太人对自己的信心。

Histology:The study of the structure of tissues by means of special staining techniques combined with light and electron microcopy.

组织学：以特殊的染色技术以及光学显微镜和电子显微镜检查、研究组织结构的学科。

The deposited films were characterized by X-ray diffraction, atomic force microscopy and field emission scanning electron microcopy to investigate the influence of the buffer layer on the crystalline quality and surface morphology.

采用X射线衍射仪、原子力显微镜和场发射扫描电子显微镜研究了缓冲层对薄膜结晶特性和表面形貌的影响。

The microstructure and composition of the coating were investigated by optical microscopy, X-ray, scanning electron microcopy. It is found that the Fe 3 Al inter-metallic compound coating can be synthesize in-situ during the coating and PTA cladding process.

通过金相显微镜、X-ray衍射、扫描电子显微镜等手段对原位合成的Fe_3Al金属间化合物涂层的组织结构和性能的研究后认为，利用上述方法能够原位合成制备Fe_3Al金属间化合物涂层。

］ In order to study the effect of machining processes on the surface condition of fastening holes drilled on 7075 aluminum alloy for aircraft assembly, the surface microphotograph and surface layers microstructure produced by the five kinds of drilling processes were observed and analyzed by means of SEM and optical microscope.

摘 要］为研究加工工艺对7075-T7351铝合金飞机装配紧固孔表面质量的影响，通过扫描电子显微镜和光学显微镜，分别对5种不同制孔工艺所产生的表面微观形貌和表层组织变化进行了观察和分析。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。