电子显微镜

The development of phase separation were followed by optical microscopy, scanning electron microscopy, transmission electronic microscopy, Synchrotron Radiation soft X-ray contact microscopy, time resolved light scattering, differential scanning calorimeters and rheometer.

本论文系统研究热塑性树脂改性环氧体系相分离过程中的流变行为，通过光学显微镜，扫描电子显微镜，透射电子显微镜，软X射线显微术，时间分辨激光光散射和DSC等仪器跟踪研究相分离过程，与体系的流变行为相对照，探究反应诱导粘弹相分离过程中流变行为。

In this work the rheological behaviors during phase separation in thermoplastic modified epoxy resin were systemically investigated. The development of phase separation were followed by optical microscopy, scanning electron microscopy, transmission electronic microscopy, Synchrotron Radiation soft X-ray contact microscopy, time resolved light scattering, differential scanning calorimeters and rheometer.

本论文系统研究热塑性树脂改性环氧体系相分离过程中的流变行为，通过光学显微镜，扫描电子显微镜，透射电子显微镜，软X射线显微术，时间分辨激光光散射和DSC等仪器跟踪研究相分离过程，与体系的流变行为相对照，探究反应诱导粘弹相分离过程中流变行为。

The camera length of electron diffraction in transmisson electron microscopy is one of the main technical parameters in designing electron microscope and the electron diffraction analysis to microcrystal sample.

透射式电子显微镜(Transm isson E lectron M icroscopy，TEM)中的电子衍射相机长度，是电子显微镜设计和对微晶体样品进行电子衍射分析的主要技术参数之一。

High-speed electronics than the wavelength of visible light wave length (wave-particle duality), while the microscope resolution by its restrictions on the use of wavelength, so the resolution of electron microscopy (about 0.1 nm) is much higher than the resolution of optical microscopy (about 200 nm).

高速的电子的波长比可见光的波长短，而显微镜的分辨率受其使用的波长的限制，因此电子显微镜的分辨率（约0.1纳米）远高于光学显微镜的分辨率（约200纳米）。

Titanium plate first was cleaned by glow Discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to the free terminal NH2 groups using glutaraldehyde as a coupling agent. Surface morphologies were observed by scanning electron microscopy and atomic force microscopy.

样本以扫描式电子显微镜及原子力显微镜﹙AFM﹚观察表面形态变化，以能量分散光谱仪进行表面元素变化之定性分析，使用X光光电子能谱仪检测表面化学键结与元素变化作定性分析与半定量分析，并以胶样金免疫标示法配合使用场发射枪扫描式电子显微镜(Field Emission Scanning Electron Microscope， FEG SEM)，观察钛金属表面白蛋白连接情形作定性分析与定量分析。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明：①ConA处理后，倒置显微镜下可见Mφ粘附性增强；扫描电子显微镜下见其体积增大，指状突起增多；透射电子显微镜下见Mφ内线粒体等细胞器增多，伸出伪足包绕结核分枝杆菌并内吞，ConA活化的Mφ吞噬结核分枝杆菌数量增多，菌体的完整性受损。

The ontogeny, phylogeny and ultrastruct of the glandular hairs of 5 varieties and 38 species in 26 genera of Labiatae are studied with the light microscopy, the scanning electron microscopy and the transmission electron microscopy in the paper.

本文利用光学显微镜、扫描电子显微镜和透射电子显微镜对唇形科26属38种和5变种的腺毛进行了个体发育、系统发育和超微结构的研究。

Surface waxes of the pericarp were observed by scanning electron microscopy; the structure and composition of cell wall were studied using light microscopy, transmission electron microscopy and Fourier transform infrared microspectroscopy respectively; the changes of calcium in the cell wall were determined by atomic absorption and the integrity of cell membrane by conductivity meter respectively.

本论文采用扫描电子显微镜研究果实表皮蜡层的变化，用光学显微镜、透射电子显微镜和傅里叶变换红外光谱仪研究果实细胞壁结构和成分的变化，用原子吸收分光光度计测定细胞壁钙离子的变化，用电导率仪检测果实细胞的完整性。同时测定了果实酚类物质含量、多酚氧化酶(EC 1.10.3.1)活性、过氧化物酶(EC 1.11.1.7)活性，并分析了果实硬度、糖和酸含量等品质指标。

The camera length of electron diffraction in transmisson electron microscopy is one of the main technical parameters in designing electron microscope and the electron diffraction analysis to microcrystal sample. According to Bragg law, the formula of calculating TEM electron diffraction camera length is derived from the research on the ray path of electron diffraction images in TEM and the comparison on electron diffraction with ordinary electronic diffractometer. The difference of physical significance of electron diffraction camera length between TEM and ordinary electronic diffractometer is discussed.

透射式电子显微镜(Transmisson Electron Microcopy， TEM)中的电子衍射相机长度，是电子显微镜设计和对微晶体样品进行电子衍射分析的主要技术参数之一依据布拉格定律，经对TEM中电子衍射成像光路的探讨与研究，并通过TEM与普通电子衍射仪的电子衍射的对比分析，导出了TEM电子衍射相机长度的精确计算公式，阐述了TEM和普通电子衍射仪的电子衍射相机长度所表征的物理意义的区别。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。