电场强度

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

In addition, we also find that the wavelength of the hexagonal wave pattern increases with 0 E which is predicted in linear stability analysis.

进一步的研究发现，点状六边形斑图的波长随着电场强度的增大而增大，这个结果和线性稳定性分析的结果定性的吻合。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

We discuss this method in detail and the role of it in the investigation of the optical property of the cholesteric liquid crystal.

研究了线性偏振光在螺旋液晶中的传播特性，并给出了光经过螺旋液晶后电场强度的矩阵表达式。

Microcomputer is in real time controlling the electrolytical field at the electro-chemical dissolution spot of workpiece,which is scanning over the workpiece surface during the machining process, so that the rate of metal removal is varying during the scanning, and the required geometry of the workpiece can be obtained.

可控电解珩磨是以微机实时控制电解珩磨过程中工件表面的电解电场强度，从而调控各点的金属去除速度，获得工件要求几何形状精度的新加工方法。

The effects of the external electric field on the hydrogenic impurity states in the GaAs quantum wire with a finite confining potential are studied by a variational method.

给出了杂质态结合能在不同的电场强度下随量子线半径、杂质位置的变化关系。

The bonding energy of a lateritic soil was chan...

作者根据实验材料认为，土壤体系中局部的电场强度较整个体系的平均体积电荷密度在决定离子的结合能方面具有更大意义；在 pH 低时，氢铝离子对交换点的竞争是钾、钠离子的结合能减小的一个重要原因。

It has been found that the cation exchange on diffusivity may play the role in different ways for different zeolites. When the diameter of the diffusant molecule is much smaller than the pore or cage size of the zeolite, the influence of exchanged cations is mainly by way of altering the electric field intensity (or the density of electric charge), the large the Z/r of the exchanged cations, the smaller the diffusivity; while the diameter of the molecule is apporsi- mately equal to the pore or channel size of the zeolite, the exchanged cations may affect the diffusivity by means of blocking up or narrowing the channels of the zeolite.

金属阳离子交换对扩散的影响表现为：当扩散分子的直径比分子筛孔道直径小得多时，交换阳离子以改变分子筛内部电场强度或电荷密度的方式影响扩散速度，阳离子的Z/r越大，扩散系数越小；当扩散分子直径与分子筛孔道直径相近时，交换阳离子则因堵塞或窄化孔道而改变分子的扩散速度。

Treated with this intensity,HVEF could increase the weight of mice and the activities of SOD and GSH-Px in both cardiac muscle cells and hepatocytes, and decreased the content of LPO, compared with control group, the difference was significant (P.01). HVEF pro moted the metabolism by regulating the activities of various enzymes.

在这个电场强度处理下，小鼠体重明显增加；心肌细胞和肝细胞中的超氧化物歧化酶、谷胱甘肽过氧化物酶的活性升高；脂质过氧化物的含量下降，与CK组比较差异有显著性（P.01）；心脏和肝脏多种生化酶指标均正常。

In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.

为了研究苜蓿中华根瘤菌电转化的条件，通过对不同的细胞生长周期、复苏缓冲液、电转缓冲液、电场强度和电阻对电转率影响程度来作出变化曲线，以确定电转化的最优条件，从而提高苜蓿中华根瘤菌电转化率。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。