甲醇

The experimental conditions were as follows: C18 chromatographic column, mobile phase of MeOH-H2O (both include 0.1% methanoic acid), flow rate at 1.0 mL/min, gradient elution, detection wavelength at 290 nm and room temperature.

采用C18色谱柱，甲醇－水（含0.1％的甲酸）梯度洗脱；流速为1.0mL/min，检测波长290nm；柱温为室温。

Saturated fatty acids and high content steroids were found in the extracts of methanolysis products.

在盐酸甲醇解产物中，主要检测到系列饱和脂肪酸和高含量的甾醇成分。

Tert-amyl alcohol was used as a reaction medium for Novozym 435 lipase-catalyzed methanolysis of soybean oil for biodiesel production.

叔戊醇作为反应介质，固定化脂肪酶Novozym 435催化大豆油与甲醇的转酯反应制备生物柴油。

Firstly, the methanolysis of different oils by alkali-catalysis and lipase-catalysis werestudied.

首先，本文分别采用酶法和化学法对不同来源的油脂和甲醇转酯化反应进行了考察和优化，对不同催化剂催化生产生物柴油进行了对比。

Results showed that AOT reversed micellar systems provided a suitable micro-environment for biodiesel production. The optimal parameters were: the ratio of water and AOT 11, AOT concentration 50 mmol/L, temperature 40℃, pH 7, molar ratio of methano and oil 3, and rotating speed 180 r/min. The transesterification rate reached 90% when a 3-step methanolysis protocol was used.

研究结果表明： AOT/异辛烷反胶束体系为Candida sp.99-125脂肪酶催化提供了较为合适的微环境，在W0为11，表面活性剂浓度为50 mmol/L，温度为40℃，缓冲液pH值为7的AOT/异辛烷反胶束体系中，醇油摩尔比为3∶1，摇床转速为180 r/min，采用12h3次流加1 mol当量的甲醇，单批最高酯转化率可以达到90%。

Jatropha curcas L.seed oil ; methanolysis ; reaction system ; phase diagram

麻疯树籽油；甲醇解；反应体系；相图

This paper focuses on the stability study of Rhizopus oryzae IFO4697 when used for the methanolysis of renewable oils for biodiesel production. The results showed that water content was important for achieving high catalytic activity and good stability of the biocatalyst. The optimum water content was found to be 5%-15%. Both particle size and desiccation methods showed no obvious effect on the stability of the biocatalyst. With GA cross-linking pretreatment, the stability of the biocatalyst could be improved significantly. When Rhizopus oryzae IFO4697 repeatedly used for next batch reaction, direct vacuum filtration was found to be a good way for the maintenance of good stability of the biocatalyst.

本实验对固定化全细胞Rhizopus oryzae IFO4697催化植物油脂合成生物柴油的稳定性进行了系统地研究，结果表明：反应体系水含量对于全细胞催化剂的反应活性和催化稳定性有重要影响， 5%~15%含水量适宜；研究范围内，载体粒度及干燥方式对稳定性影响不显著；经过戊二醛交联后，全细胞催化油脂甲醇解反应的稳定性显著提高， 1200 h反应后，仍然可以保持75%的生物柴油得率；真空抽滤直接回用的方式有利于稳定性的保持。

The oil was further explored for biodiesel production by lipase-catalyzed methanolysis. Two processes were studied, namely, three-step methanol loading in solvent-free system and all-in-one methanol loading in tert-butanol system.

进一步利用该酵母油脂为原料分别研究了无溶剂体系中三步甲醇法及在叔丁醇介质体系中脂肪酶催化合成生物柴油，发现脂肪酶可以有效转化该酵母油脂制备生物柴油。

The density functional theory and MP2 method were employed to study the reaction pathways of methanolysis of 3-methyl cyclic ethylene phosphate: CH3O－＋MEP; CH3OH＋MEP; CH3O－＋HMEP; CH3OH＋HMEP.

采用密度泛函理论和MP2方法研究了3-甲基环状乙撑磷酸二酯与甲醇的反应途径： CH3O－＋MEP； CH3OH＋MEP； CH3O－＋HMEP； CH3OH＋HMEP。

The free fatty acids were extracted with petroleum ether and were separated on silicon gel column chromatography. The ethyl acetate-soluble lipid fraction and water-soluble lipid fraction were subjected to hydrochloric acid-catalyzed methanolysis, then were extracted with petroleum ether, respectively.

用石油醚提取低极性组分，经硅胶柱层析分离得到游离脂肪酸；用乙酸乙酯提取酯溶性神经酰胺及其衍生脂类，提取物和残余的水溶性脂类分别经盐酸甲醇解，水解产物再用石油醚提取。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。