甲蓝

Following studies had been performed:(1)repetitive WLT and real-time ultrasonography had been performed to 18 HS and 40 FD toassess the reproducibility and influencing factors.(2) correlations of WLT with symptomscore and electrogastrography had been analysed.(3) differences ofWLT between HS and FD had been compared.(4) methophenolane andimmunohistochemistry stain of gastric fundus mucosa biopsy from 40 FD patients hadbeen performed to analyse the correlations of symptom score and WLT with count andactivity of mast cells, 5-HT level, and H.Pylori infectious status.(5) the effect of oralsumatriptan to proximal stomach function in 10 HS and 10 FD had been assessed, so as toestablish the possible regulating mechanism of fundic relaxation, explore the therapeuticpotential and feasibility of sumatriptan on FD.Results:I. Evaluation of WLT and its application in FD management.

我们主要进行以下几个方面的研究：(1)对18名健康人及40名FD患者重复进行水负荷试验并结合B超声对近端胃的实时监测，评价水负荷试验结果的可靠性并分析其影响因素；(2)观察水负荷试验结果与症状积分及体表胃电结果的相关性；(3)比较FD患者和健康成人水负荷试验结果的差别；(4)对40名FD患者胃底粘膜活检组织进行免疫组化染色和甲苯胺蓝染色，观察FD患者的症状及水负荷试验结果与胃底粘膜肥大细胞、5-HT表达情况及H.pylori感染情况的相关性：(5)观察口服舒马曲坦对10名健康人及10名FD患者近端胃功能的影响，并由此推测胃底舒张运动调节的生理机制，探讨口服舒马曲坦治疗FD的可行性。

Staining observation on complex mesochondrium of marrow-derived mesenchymal stem cells-TCP: It was found with TB staining that the complex of marrow-derived mesenchymal stem cells-TCP inside the joint cavity obviously had positive staining, which indicated that there were glycosidoprotein base formed.

骨髓基质干细胞-磷酸三钙复合体软骨基质特染观察结果：甲苯胺蓝染色发现，植入关节腔的骨髓基质干细胞-磷酸三钙复合体有明显阳性染色，表明有糖蛋白基质形成。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红&O&染色呈强阳性。

Then the bFGF was replaced by transforming growth factor-β1 (TGF-β1 10 μg·L-1) for next three weeks. Results Some spindle MSCs turned into round or elliptic shape and secreted metachromatic matrix with Toluidine blue.

结果：细胞形态由纺锤形变为圆形或椭圆形，细胞分泌了大量的甲苯胺蓝异染的基质，形成了类软骨陷窝。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标：倒置相差显微镜下观察骨髓间充质干细胞的生长状态，流式细胞仪检测细胞表面标志物的表达，采用碱性磷酸酶染色鉴定成骨能力，以油红O染色鉴定成脂能力，以甲苯胺蓝染色鉴定成软骨能力。

Subchondral bone depicted high-signal intensity on FSE-T〓WI and STIR in 10 cases.In histopathologic examine, pannus was infiltrating the articular cartilage during 10 to 31 days after injection and monocuclear leukocyte infiltration was found.

关节软骨甲苯胺蓝染色切片显示，注入菌液7天后开始，随化脓性炎症时间的延长，关节软骨的染色逐渐变浅，且以表层及中层的软骨染色变浅为明显，而底层软骨染色深度较表层及中层深。

The interaction ratio and constant were calculated by the voltammetric data.4. Studies on the interaction of phenosafranine, toluidine blue with hyaluronic acid.The interaction of PSF and TB with HA were studied by liner sweep voltammetry. The conditions for the interaction were optimized. The working curve for HA was established under the optimal conditions.

考察了有机染料酚藏花红、甲苯胺蓝与透明质酸的相互作用，优化了结合反应条件，在最佳实验条件下绘制了测定透明质酸的工作曲线并成功用于透明质酸模拟样品的测定，结果令人满意。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果：苏木精－伊红染色细胞多为梭形，有伪足伸出，细胞核为圆形或椭圆形；甲苯胺蓝染色，胞浆为深蓝色；免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达；在体积分数为0.1的血清条件下，胰岛素样生长因子Ⅰ能提高细胞的增殖活性，并且在有效浓度范围内呈剂量效应关系。

Methods This study contained a total of 16 culture groups, including one group as control and 5 groups treated by 5 drugs (calcium L-threonate, sodium L-threonate, alendronate, 17β-estradiol and calcium gluconate) each at the final concentrations of 10^(-9)mol/L, 10^(-7)mol/L, 10^(-5)mo1/L respectively. After 7 days, eight bone slices of every group were stained with toluidine blue and the areas of resorptive pits were analyzed under light microscope; the concentrations of C-telopeptide of type Ⅰ collagen CT(subscript x or Crosslaps in culture supernatants were measured by ELISA.

制备骨磨片，培养OC，并分别加入高中低浓度10^(-5、10^(-7)、10^(-9)mol/L的L-苏糖酸钙、L-苏糖酸钠、阿仑麟酸钠、17β-雌二醇、葡萄糖酸钙，实验设以上各浓度组及对照组（共16组，每组n=8），甲苯胺蓝染色一光镜观察分析骨片上骨吸收陷窝面积，ELISA测定上清液Ⅰ型胶原C-末端肽（CTx或Crosslaps）浓度。

It suggests that the supraspinous ligaments in the patients with ossification of the ligament flavum also have an obvious tendency of ossification.

结果：胸椎黄韧带骨化症患者棘上韧带内胶原纤维增生、肿胀，出现了大量的纤维软骨细胞，甲苯胺蓝染色呈强异染性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。