甲蓝

Chicken, Chicken Meal, Whole Ground Brown Rice, Whole Ground Barley, Chicken Liver, Pea Fiber, Chicken Fat (Preserved with Natural Mixed Tocopherols and Citric Acid), Brewers Dried Yeast, Natural Chicken Flavor, Dried Egg Product, Oil Blend (Soybean oil, Olive Oil, Salmon Oil, Evening Primrose Oil; Preserved with Natural Mixed Tocopherols and Citric Acid), Pumpkin, Apples, Sweet Potatoes, Carrots, Spinach, Blueberries, Dried Cranberries, Clams, Whole Ground Flaxseed, Calcium Carbonate, Salt, Potassium Chloride, Taurine, Green Lipped Mussels, Dried Chicory Root Extract, Yucca schidigera extract, Grape Seed Extract, Dried Kelp, L-Ascorbyl-2-Polyphosphate, Glucosamine Hydrochloride, Chondroitin Sulfate, Vitamin E Supplement, Ferrous Sulfate, DL-Methionine, Zinc Proteinate, Zinc Oxide, Manganese Proteinate, Niacin, Copper Proteinate, Folic Acid, Vitamin B12 Supplement, Copper Sulfate, Manganous Oxide, Vitamin A Supplement, Sodium Selenite, Thiamine Mononitrat Calcium Pantothenate, Riboflavin, Pyridoxine Hydrochloride, Biotin, Vitamin D3 Supplement, Menadione Sodium Bisulfite Complex, Calcium Iodate, Dried Lactobacillus acidophilus Fermentation Solubles, Dried Lactobacillus lactis Fermentation Solubles, Dried lactobacillus casei Fermentation Solubles, Rosemary Extract.

创造了一种全新的生产模式：低温焗炉焗制，低温焗烤不仅保存了食物中的营养和矿物质，而且降低了油份，让您的宠物食得更健康。更值得一提的是，通过数年投入大量人力、财力经过不断的尝试，终于于2009年年初研制出适合猫咪的最好食粮，无论从口感、成分配比，营养分析等综合因素，是您可以选择的最好的食材。鸡肉，鸡肉粉，全糙米，全地面大麦，鸡肝，豌豆纤维，鸡脂肪（天然保存溷合生育酚和柠檬酸），干酵母，天然鸡肉香精，干蛋产品，溷合油（豆油，橄榄油，三文鱼油，月见草油，天然溷合生育酚和柠檬酸保存），南瓜，苹果，红薯，胡萝卜，菠菜，蓝莓，小红莓干，蛤，全亚麻籽，碳酸钙，盐，氯化钾，牛磺酸，绿唇贻贝，干菊苣根提取物，丝兰提取物，葡萄籽提取物，干海带，L -抗坏血酸- 2 -聚，氨基葡萄糖盐酸盐，硫酸软骨素，维生素E补充，硫酸亚铁，DL -蛋氨酸，锌蛋白盐，氧化锌，锰蛋白盐，烟酸，铜蛋白盐，叶酸，维生素B12，硫酸铜，氧化锰，维生素A补充剂，亚硒酸钠，硫胺Mononitrat泛酸钙，核黄素，盐酸吡哆醇，生物素，维生素D3的补充，亚硫酸氢钠甲萘醌复合，碘酸钙，干嗜酸乳杆菌发酵米糠，干乳酸乳杆菌萃取物，干乳杆菌萃取物，迷迭香提取物。

The histological staining showed that toluidine blue, safranin O and anti-collagen II immunohistochemistry staining were positive.

组织学观察显示，三维多孔支架甲苯胺蓝染色、番红O染色、Ⅱ型胶原免疫组织化学染色均呈阳性。

NBT and safranin O were used to stain the sample. The interaction between PMNs and the parasites was observed under microscope.

用氮蓝四唑和沙黄O染色，显微镜观察中性粒细胞与阴道毛滴虫相互作用及甲臢（NBT还原产物颗粒沉积。

The effect of Alcian blue Safranin O staining was the best, which was applicable to identification of the mast cell of A. Japonica. The improved toluidine blue staining was not applicable.

AB/SO染色法效果最好，适用于日本鳗鲡肥大细胞的鉴定；改良甲苯胺蓝染色法不适合于日本鳗鲡肥大细胞的鉴定。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好；移植皮下后第4周取材可见外形呈类骺软骨样组织块，行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色，镜下观察可见大量软骨细胞及类软骨陷窝样结构，植入的海藻酸钙微球周围组织无明显炎症反应。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

At day 7, 14 and 21 after induction respectively, Toluidine blue staining and immunocytochemical staining were performed to detect differentiation.

在诱导第7，14，21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups.

实验组甲苯胺蓝染色和Ⅱ型胶原染色为阳性，对照组和空白组均为阴性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。