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甲苯胺红

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华派公司产品:对氯苯酚,邻氯苯酚,2,4二氯苯酚,2,6二氯苯酚,2,4,5三氯苯酚,2,4,6三氯苯酚,4氯2苄基苯酚,对溴苯酚,2,4,6三溴苯酚,对氯苯基叔丁基醚,间溴苯甲醚,间溴氟苯,α溴甲基四氢呋喃,α溴代4氯苯乙酮,α溴4甲氧基苯乙酮,4氯二苯甲酮,4氯2甲基苯胺,N(2羟乙基)乙二胺, 2,3二溴丙酰氯,对溴苯磺酰氯,对苯二甲酰氯,间苯二甲酰氯,N氯代丁二酰亚胺,N溴代丁二酰亚胺,四氯苯醌,5氯靛红。

The lung was fixed in 10% formalin solution and embedded in paraffin for Haematoxyffin-eosin staining to observed the form of embolisms, Masson staining to assess collagen and fibrin/fibrinogen distribution and Toluidine blue staining was to observe mast cell distribution.

以10%甲醛固定肺组织,制成组织切片,行苏木精-伊红染色观察血栓的形态,Masson染色观察栓塞血管的胶原纤维分布,甲苯胺蓝染色观察血管栓塞处肥大细胞分布。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间,进行髓核细胞活力测定;爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察;MTT 法绘制髓核细胞生长曲线,并行原代及第2 代细胞透射电镜观察,对体外细胞的生物学特性进行研究。

At the third passage, BMSCs showed the typical polar swirl morphology. BMSCs were negative for CD34 and CD45, but positive for CD29 and CD44. Following induction, alkaline phosphatase staining, Oil red staining and toluidine blue staining produced a strong reaction in cells.

第3代骨髓间充质干细胞形态单一均匀,呈典型的极性漩涡状生长,不表达造血前体细胞标志抗原CD34和白细胞标志抗原CD45,表达整合素家族成员CD29和黏附分子CD44,经诱导后碱性磷酸酶染色、油红O染色和甲苯胺蓝染色均呈阳性。

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